Ratprimaryantibodiesonmousetissuesareadvantageousbecauseratsecondarydetectionsystemscanbeusedwithminimumcross-reactivitytoendogenousmouseIgG.BiocareMedicalhasdevelopedaRat-on-Mousealkalinephosphatase(AP)polymerdetectionsystemwithminimalcross-reactivitytomousetissues.Itcanbeusedwithformalin-fixedparaffin-embeddedtissues,floatingsections,frozensectionsandcellculturepreparations.TheRat-on-MouseAP-Polymeriscomprisedoftworeagents:aRatProbethatbindstotheratprimaryantibodyandaRat-on-MouseAP-Polymerthatconjugatestotheprobe.Thistwo-stepsystemhasproventobe10-20timesmoresensitivethanconventionalconjugatedmouseadsorbedanti-ratAPsecondarydetectionsystems.BiocareMedicalhasnovelproductstohelpeliminatepersistentmouseIgG.XMFactorisaverypotentblockerforeliminatingendogenousmouseIgG.Byadding1-2drops(1dropequals33µl)oftheXMFactorto2.5mloftheRat-on-MousePolymer,endogenousmouseIgGwillbedramaticallyreducedand/orcompletelyeliminated.RodentBlockMisablockingreagentfornonspecificbackgroundstainingthatalsoreducesendogenousmouseIgG.RodentBlockMisappliedpriortotheprimaryantibodyfor15-30minutes.BiocareMedicalhasalsodevelopedRodentDecloaker,anantigenretrievalsolution,thathelpsreduceand/oreliminateendogenousmouseandratIgGandnon-specificbackgroundstaining.TemperaturedependentprotocolscanbeperformedusingBiocare’sDecloakingChamber.
