Biocare’sPromARKTMseriesintroducesRabbit-on-FarmaHRP-Polymerdetection.TheRabbit-on-FarmaHRP-Polymerisformulatedtodetectrabbitpolyclonalormonoclonalantibodiesandisaffinitypurifiedforminimalcross-reactivitytocow,horse,pigandsheepIgGproteins.Theadvantagesofthispolymertechnologyareincreasedsensitivity,reductionofIHCsteps(noavidin/biotinblockorlink/enzymeconjugates),minimalcross-reactivitytoendogenousIgGthatallowsmultiplespeciesflexibility,andinmostcases,tissuesdonotrequireaproteinblock.TheRabbit-on-Farmadetectioncanbeusedonparaffin-embeddedtissuesandcanbeperformedmanuallyoronautomatedstainingplatforms.ThisdetectionmaybeusedwithanyofBiocareMedical’sretrievalsolutions(Reveal/Borg/Diva)ordigestiveenzymes.TemperaturedependentprotocolscanbeconvenientlyperformedusingBiocare’sDecloakingChamber.TheRabbit-on-FarmaHRP-PolymermaybecombinedwiththeMouse-on-FarmaHRP-PolymertoprepareauniversalHRP-PolymerdetectionthatwilllabelbothmouseandrabbitantibodieswithHRP.RefertoTechnicalNote#4foradditionalinformation.TheRabbit-on-FarmaHRP-PolymermaybecombinedwiththeMouse-on-FarmaAP-PolymertoprepareadoublestainpolymerdetectionthatwilllabeltherabbitantibodywithHRPandthemouseantibodywithAP.RefertoTechnicalNote#5foradditionalinformation.
