Rabbit-on-RodentAP-Polymerhasbeendevelopedforusewithrabbitmonoclonal/polyclonalantibodiesonmouseandrattissues.Rabbitprimaryantibodiesonmouseandrattissuesareadvantageousbecauserabbitsecondarydetectionsystemscanbeusedwithminimumcross-reactivitytoendogenousmouseandratIgG.AdvantagesofBiocareMedical’spolymertechnologyareincreasedsensitivity,reductionofIHCstepsandeliminationofendogenousbiotin.Itcanbeusedwithformalin-fixedparaffin-embeddedtissues,floatingsections,frozensectionsorcellcultures.Biocare’sRabbit-on-RodentAP-Polymerisidealforsinglestainingandmayalsobeusedindoubleandtriplestainingtechniques.Insometissuessuchasspleen,endogenousmouseorratIgGmaybedifficulttoeliminate.BiocareMedicalhasdevelopedanantigenretrievalsolution(RodentDecloaker)thathelpsreduceoreliminateendogenousIgGandnon-specificbackgroundstainingwhilesimultaneously,performingantigenretrieval.Temperaturedependentprotocolsat80ºC,95ºCand125ºCcanbeperformedusingBiocare’sDecloakingChamber.BiocareMedicalhasadditionalproductstohelpeliminatepersistentratandmouseIgG.TheXMandXRFactorsaretwopotentblockersforeliminatingendogenousmouseorratIgG.Byadding1-2drops(1dropequals33µl)oftheXMorXRFactorto2.5mlofRabbit-on-RodentAP-Polymerdetection,endogenousmouseorratIgGwillbereduceddramaticallyorcompletelyeliminated.InadditionRodentBlockM(mousetissue)andRodentBlockR(rattissue)blockfornon-specificbackgroundstainingandreducestainingduetoendogenousmouseorratIgG.RodentBlockMorRisappliedtothetissuepriortotheprimaryantibodyfor15to30minutes.
