CK5andCK14arehighmolecularweightcytokeratinsexpressedinavarietyofnormalandneoplasticepithelialtissues(1).Inprostatetissue,mRNAforCK5andCK14hasbeendetectedinthebasalcellsofnormalglandsandprostaticintraepithelialneoplasia(PIN),aprecursorlesiontoprostaticadenocarcinoma;however,expressionofCK5orCK14wasnotidentifiedininvasiveprostaticadenocarcinoma(2).
p63,ahomologofthetumorsuppressorp53,hasbeenidentifiedinproliferatingbasalcellsintheepitheliallayersofavarietyoftissues,includingepidermis,cervix,urotheliumandprostate(3).p63wasdetectedinnucleiofthebasalepitheliuminnormalprostateglands;however,itwasnotexpressedinmalignanttumorsoftheprostate(4).
| Format | VP |
|---|---|
| Volume | 6.0ml |
| IntendedUse | IVD |
| ByLetter | P |
| Antigen | CK14,CK5,p63 |
| Isotype | IgG1/kappa,IgG2a/kappa,IgG3 |
| Source | MouseMonoclonal |
References:
1.MollR,etal.Thecatalogofhumancytokeratins:patternsofexpressioninnormalepithelia,tumorsandculturescells.Cell.1982Nov;31(1):11-24.
2.YangY,etal.differentialexpressionofcytokeratinmRNAandproteininnormalprostate,prostaticintraepithelialneoplasia,andinvasivecarcinoma.AmJPathol.1997Feb;150(2):693-704.
3.YangA,etal.p63,ap53homologat3q27–29,encodesmultipleproductswithtransactivating,death-inducing,anddominant-negativeactivities.MolCell.1998Sep;2(3):305-16.
4.SignorettiS,etal.p63isaprostatebasalcellMarkerandisrequiredforprostatedevelopment.AmJPathol.2000Dec;157(6):1769-75.
5.TachaDE,MillerRT.Useofp63/P504Smonoclonalantibodycocktailinimmunohistochemicalstainingofprostatetissue.ApplImmunohistochemMolMorphol.2004Mar;12(1):75-8.BiocareMedical,WalnutCreek,California.
6.BeachR,etal.P504Simmunohistochemicaldetectionin405prostaticspecimensincluding37618-gaugeneedlebiopsies.AmJSurgPathol.2002Dec;26(12):1588-96.
7.LuoJ,etal.Alpha-methylacyl-CoAracemase:anewmolecularmarkerforprostatecancer.CancerRes.2002Apr15;62(8):2220-6.
8.WangY,etal.Celldifferentiationlineageintheprostate.Differentiation.2001Oct;68(4-5):270-9.
9.TokarEJ,etal.Stem/Progenitorandintermediatecelltypesandtheoriginofhumanprostatecancer.Differentiation.2005Dec;73(9-10):463-73.
10.CollinsAT,etal.Identificationandisolationofhumanprostateepithelialstemcellsbasedonalpha(2)beta(1)-integrinexpression.JCellSci.2001Nov;114(Pt21):3865-72.
11.CenterforDiseaseControlManual.Guide:SafetyManagement,NO.CDC-22,Atlanta,GA.April30,1976“DecontaminationofLaboratorySinkDrainstoRemoveAzideSalts.”
12.ClinicalandLaboratoryStandardsInstitute(CLSI).ProtectionofLaboratoryWorkersfromOccupationallyAcquiredInfections;ApprovedGuideline-FourthEditionCLSIdocumentM29-A4Wayne,PA2014.
