Mousemonoclonalantibodiescanbeusedforimmunohistochemicaldetectiononmousetissues;however,thebiotinylatedsecondaryantibodyusedfordetectionoftheprimaryantibodywillalsobindtotheendogenousmouseIgGinthetissue,resultinginbackgroundstainingfrombound-mouseIgG.Tocircumventtheproblem,BiocarehasdevelopedtheMMBiotinylationKit.Thekitprovidesallthereagentsnecessarytobiotinylateaprimaryantibody.

Thebasicconceptofthekitisverysimple.Theantibodyismixedwithabiotinylationreagentfor30minutespriortotheapplicationofprimaryantibody.Aslittleas5to10µLofantibodycanbeused.Oncethecomplexisformed,amoppingreagentisaddedandthenthebiotinylatedantibodycomplexisappliedtothetissuespecimen.Thetissueisthenincubatedwiththestrepavidin-peroxidasereagent,andthecolorreactionisdevelopedbyaDABchromogensubstrate.

ThesystemhasbeenspeciallyformulatedtoeliminateendogenousmouseIgGbackgroundstaining.Eventhemostdifficulttissuessuchaslymph,spleen,lungandkidneyarevirtuallybackgroundfree.

EachKitIncludes:
1.DaVinciGreen(PD900H)
2.BackgroundSniper(BS966H)
3.Peroxidazed1(PX968H)
4.BiotinylationReagent(MMBR610F)
5.MoppingReagent(MMMR611F)
6.Streptavidin-HRPLabel(HP604H)
7.DABChromogen(DB851D)
8.DABSubstrateBuffer(DS854H)
9.CATHematoxylin(CATHEH)
10.DABSparkle(DS830H)
11.MixingVial(VL103)