Theconjugatedgoatanti-mouseandanti-rabbitpolymer-horserADIshperoxidasesecondaryantibodyreactswithheavyandlightchainsonmouseandrabbitIgG.ItreactswithallmouseandrabbitIgGsubclassesandwithmouseandrabbitIgM.ThenewandinnovativeHRP-polymerizationtechnologyprovidesasignificantincreaseinstainingsensitivitywhencomparedtootherconventionalHRP-conjugatedsecondaryantibodies.Avidin-biotinblockingproceduresarenotnecessarywhenusingtheMACH2conjugatedsecondaryantibodies.Theoverallstainingprocedureusesonelessreagent(streptavidinperoxidase)andrinsingstep,eliminatesavidin-biotinblockingprocedures,andmayreduceoverallstainingtime.Itcanbeusedmanuallyandonautomatedstainers.