Theconjugatedgoatanti-mousepolymer-alkalinephosphatase(AP)secondaryantibodyreactswithheavyandlightchainsonmouseIgG.ItreactswithallmouseIgGsubclassesandwithmouseIgM.TheinnovativeAP-polymerizationtechnologyprovidesasignificantincreaseinstainingsensitivitywhencomparedtootherconventionalAP-conjugatedsecondaryantibodies.Avidin-biotinblockingproceduresarenotnecessarywhenusingtheMACH2conjugatedsecondaryantibodies.Theoverallstainingprocedureusesonelessreagent(streptavidinperoxidase)andrinsingstep,eliminatesavidin-biotinblockingprocedures,andmayreduceoverallstainingtime.Itcanbeusedmanuallyandonautomatedstainers.