Theconjugatedgoatanti-mousepolymeralkalinephosphatase(AP)andtheconjugatedgoatanti-rabbitpolymerhorserADIshperoxidase(HRP)secondaryantibodiesreactwithbothheavyandlightchainsonmouseandrabbitIgGrespectively.ThisinnovativeAP-andHRP-polymerizationtechnologyprovidesasignificantincreaseinstainingsensitivitywhencomparedtootherconventionalAP-orHRP-conjugatedsecondaryantibodies.Avidin-biotinblockingproceduresarenotnecessarywhenusingtheMACH2conjugatedsecondaryantibodies.Itisspeciallydesignedforarapiddoublestainprocedure.Anantibodycocktailwitharabbitmonoclonal/polyclonalandamousemonoclonalantibodymustbeused.Theoverallstainingprocedurecanbedonein4stepsandinlessthantwohours.Itcanbeusedmanuallyorusedwithautomatedstainers.