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Chromogenicinsituhybridization(ISH)permitsthevisualidentificationofspecificmRNAorDNAnucleicacidsequencesintissues.Followingapplicationoftheprobe,thepresenceofatargetnucleicacidisvisualizedbythesequentialapplicationofasecondaryreagentthatbindsthedigoxigeninlabeledprobe,followedbyatertiaryenzyme-antibodyconjugate,andachromogenreagent,toproduceacoloredreactionproductthatisvisIBLebylightmicroscopy.
ISHHRPDetectioniscomprisedofamouseanti-digoxigeninantibodyasasecondaryreagentandanHRPenzyme-antibodyconjugateasatertiaryreagent,suitableforthedetectionofdigoxigeninlabeledprobesaspartofanISHstainingprocedureontheONCOREAutomatedSlideStainer.ISHHRPDetectionisprovidedready-to-useandisintendedtobeappliedasdefinedbythestainingprotocolsontheONCOREAutomatedSlideStainer.
Format | ONCORE |
---|---|
IntendedUse | IVD |
Volume | 60Tests |
References:
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2.ShibataY,etal.AssessmentofdecalcifyingprotocolsforthedetectionofspecificRNAbynon-rADIoactiveinsituhybridizationincalcifiedtissues.HistochemCellBiol.2000Mar;113(3):153-9.
3.WilkinsonDG.InSituHybridization:APracticalApproach(PracticalApproachSeries).2ndEd.Oxford:OxfordUniversityPress,1999.
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5.ClinicalandLaboratoryStandardsInstitute(CLSI).ProtectionofLaboratoryWorkersfromOccupationallyAcquiredInfections;ApprovedGuideline-FourthEditionCLSIdocumentM29-A4Wayne,PA2014.