Helicobacterpylori(H.pylori)infectionrepresentsthemajorcauseofpepticulcerdiseaseandisalikelycontributingfactorinthedevelopmentofgastricneoplasticdiseasessuchasgastricadenocarcinomaandmucosa-associatedlymphoidtissue(MALT)lymphoma(1).H.pyloriarespiral-curved,gram-negativebacteria.ThemajorityofH.pylorimicroorganismsresideintheunstirredlayerofthegastricmucus,butsomebindtotheluminalaspectofgastricfoveolarcells,andattachatornearintercellularjunctions,orevenlocatewithincells(2-3).However,generallytheydonotinvadetheunderlyinglaminapropria(3).Immunohistochemical(IHC)detectionofH.pyloricanspecificallydistinguishthemicroorganismsfromothertypesofbacterialinfections.CompletestainingoftheH.pylorimicrorganismhasbeenshownwiththerabbitmonoclonal.AnIHCtechnique,withheat-induced-antigenretrievalfordetectionofH.pyloriingastricbiopsies,ishighlyreliable,highlysensitive,specific,andcanbeusedonfullyautomatedIHCplatforms.
| Format | ONCORE,Predilute |
|---|---|
| Volume | 6.0ml,60Tests |
| IntendedUse | IVD |
| Source | RabbitMonoclonal |
| SpeciesReactivity | Human;othersnottested |
| Localization | Spiralshapedbacterium |
| Clone | EP279 |
| Antigen | H.Pylori |
| PositiveControl | GastrictissueinfectedwithH.pylori |
| Isotype | IgG |
| ByLetter | H |
References:
1.DunnBE,CohenH,BlaserMJ.Helicobacterpylori.ClinMicrobiolRev.1997;10:720-41.
2.BorenT,etal.AttachmentofHelicobacterpyloritohumangastricepitheliummediatedbybloodgroupantigens.Science.1993;262:1892-5.
3.HazellSL,etal.Campylobacterpyloridisandgastritis:associationwithintercellularspacesandadaptationtoanenvironmentofmucusasimportantfactorsincolonizationofthegastricepithlium.JInfectDis.1986;153:658-63.
4.CenterforDiseaseControlManual.Guide:SafetyManagement,NO.CDC-22,Atlanta,GA.April30,1976“DecontaminationofLaboratorySinkDrainstoRemoveAzideSalts.”
5.ClinicalandLaboratoryStandardsInstitute(CLSI).ProtectionofLaboratoryWorkersfromOccupationallyAcquiredInfections;ApprovedGuideline-FourthEditionCLSIdocumentM29-A4Wayne,PA2014.
