Goat-on-RodentAlkalinePhosphatase(AP)polymerhasbeendevelopedforusewithgoatprimaryantibodiesonmouse,ratandhumantissues.Goatprimaryantibodiesareadvantageousbecausegoatsecondarydetectionsdisplayminimumcross-reactivitytoendogenousmouse,rat,andhumanIgG.AdvantagesofBiocareMedical’sbiotin-freepolymertechnologyareincreasedsensitivity,areductionofIHCstepsandeliminationofendogenousbiotin.Itcanbeusedwithformalin-fixedparaffin-embeddedtissues,floatingsections,frozensectionsorcellcultures.TheGoat-on-RodentAP-Polymeriscomprisedoftworeagents:thegoatprobethatlabelsthegoatprimaryantibodyandanAP-polymerthatconjugatestotheprobe.Thistwo-stepsystemhasbeenproventobe10-20timesmoresensitivethanconventionalmouseanti-goatAPsecondarydetectionsystems.BiocareMedicalhasdevelopedRodentBlockM(formousetissues)orRodentBlockR(forrattissues)blockingreagentsthatreducenonspecificbackgroundstainingandendogenousmouseorratIgG.RodentBlockisappliedtothetissuepriortotheprimaryantibodyfor15-30minutes.Forhumantissues,useBackgroundPunisher.BiocareMedicalhasalsodevelopedRodentDecloaker,anantigenretrievalsolution,whichhelpsreduceandeliminateendogenousmouseandratIgGandnon-specificbackgroundstainingwhilesimultaneouslyperformingantigenretrieval.Forhumantissues,pleaserefertotherespectiveprimaryantibodydatasheetforrecommendedsolutions.TemperaturedependentprotocolscanbeperformedusingBiocare’sDecloakingChamber.
