Description:
UsetheCFX96DeepWellOpticalReactionModuleforprecise,reproducIBLereal-timePCR.Theopticalmoduleincludesanopticalsystemforcollectionoffluorescentdataandathermalcyclerblock.SYBR®GreenSupermix,96-wellPCRplates,andplatesealsareincluded.
TheCFX96DeepWellOpticalReactionModuleisamemberofthefamilyofinterchangeablereactionmodulesthatcanbeusedwiththeC1000TouchThermalCyclerChassisforPCRandreal-timePCR.Allmodulescanbequicklyinsertedandremovedfromthecyclerchassiswithouttools.
FeaturesandBenefits
WhentheCFX96DeepWellOpticalReactionModuleiscombinedwiththethermalcyclerchassis,thesystemoffers:
- Deepwellsforlargevolumes
- Detectionofupto5targetsperwell,plusachanneldedicatedtosingleplexFRET
- Protocolautowriterthatgeneratesanoptimalprotocolforyourreactioncomponents
- ThermalgrADIentfeaturethatidentifiesoptimalannealingtemperatureinasinglerun
- CFXMaestro™Softwareandqbase+Softwareforreliablevalidation,dataanalysis,andexportofRDMLfilesforconformancewithMIQEguidelines
TheCFX96Touch™DeepWellReal-TimePCRDetectionSystemhassixindependentlycontrolledthermalelectricunitsprovidingeven,preciselycontrolledtemperaturesatalltimesduringtherun,includingramping.Thereducedmassofthehoneycombblockprovidesfastrampingandreducedsettlingtime(timetoachievethermaluniformity).
Theopticalsystem,withsixfilteredLEDsandsixfilteredphotodiodes,collectsdatafromallwells,detectinguptofivetargetsperwell.Forsingle-colorFAMandSYBRGreenI,thefastscanoptionreadssingle-channelfluorescenceinall96wellsinjust3seconds.AchannelwithanLEDfilter–photodiodecombinationisdedicatedtoFRETsingleplexexperiments.
Thelargetouch-screeninterfacehasintuitivegraphicalprogramminganddisplaysdatatracesinrealtime.Theprogrammablethermalgradientfeaturecantesteighttemperaturesatoncetoquicklydeterminetheoptimalannealingtemperature,andtheprotocolautowriterquicklyoptimizesreactions,generatinganoptimalprotocolbasedonyourpolymerase,primers,andproductlength.
ProductComponents
- CFX96DeepWellOpticalReactionModule
- USBcable
- CFXMaestroSoftware
- Instructionmanual
- qbase+Softwarelicense
- SsoAdvanced™UniversalSYBR®GreenSupermix(1725270)
- Hard-Shell®Low-ProfileThin-Wall96-WellSkirtedPCRPlates(HSP9601)
- Microseal®"B"AdhesiveSeals(MSB1001)
ConfigurationOptions
ThefollowingmodulescanbeusedwiththeC1000TouchThermalCyclerChassis:
| ReactionModules | CatalogNumber | |
| Module | ModulewithStarterPackage* | |
| ForPCR | ||
| Dual48/48FastReactionModule | 1840148 | — |
| 96-WellFastReactonModule | 1840196 | — |
| 96–DeepWellReactionModule | 1840197 | — |
| 384-WellReactionModule | 1840138 | — |
| ForReal-TimePCR | ||
| CFX96OpticalReactionModule | 1845097 | 1845096 |
| CFX96DeepWellOpticalReactionModule | 1844095 | 1844096 |
| CFX384™OpticalReactionModule | 1845385 | 1845384 |
*StarterpackagecontainsSsoAdvanced™UniversalSYBR®GreenSupermix(1725270),Microseal"B"AdhesiveSeals(MSB1001),andHard-ShellLow-ProfileThin-Wall96-WellSkirtedPCRPlates(HSP9601)orHard-ShellThin-Wall384-WellSkirtedPCRPlates(HSP3805).
TheCFX96TouchDeepWellReal-TimePCRDetectionSystemcanbeconfiguredwithotherPCRinstruments,allowingstand-aloneorsoftwarecontrol.InstrumentpermutationsallowuptofourdifferentCFXConnect™,CFX96,orCFX384SystemstobecontrolledbyonePC.
CombineanytwoCFXReal-TimePCRDetectionSystemswiththeCFXAutomationSystemII,ourroboticplatehandler,tocreateahigh-throughput,automatedreal-timePCRsystemwithacompactfootprint.
ServicePlansandValidationToolsforCFXSystems
- Ensurethatyourreal-timePCRsystemsarefunctioningoptimallywithBio-RadExpertCareService;choosefromseveralserviceplanstokeepyourCFXAutomationSystemIIrunningitsbest
- CertifysysteminstallationandvalidatesystemperformancewithCFXSystemIQ/OQServicesandCFXAutomationSystemIIIQ/OQServices
- VerifytheperformanceofyourCFXSystemswithThermalValidationServicesandtheCFXQualificationPlates
RelatedProducts
- C1000TouchThermalCyclerChassis(1841100)
- PX1™PCRPlateSealer
- PCRplasticsoptimizedforCFXReal-TimePCRDetectionSystems:
- Hard-Shell96-WellLowProfileSkirtedPCRPlates
- MicrosealB"AdhesiveSeals(MSB1001)
- OpticallyClearHeatSeals(1814030)
- CFXMaestroSoftware,SecurityEdition,forcompliancewithU.S.FDA21CFRPart11regulations
- CFXQualificationPlateforperformanceverification,96-well(1845098)
RelatedCategories
- Real-TimePCRDetectionSystems
- PCRReagentsandqPCRReagents
- PCRPlasticConsumables
- PCRSeals
- PCRAnalysisSoftware
SupportingDocuments
- CFX96TouchDeepWellReal-TimePCRDetectionSystemSpecificationsSheet
- CFX96TouchandCFX96TouchDeepWellReal-TimePCRDetectionSystemsInstallationQuickGuide
- CFX96Touch,CFX96TouchDeepWell,andCFX384Touch™Real-TimePCRDetectionSystemsInstructionManual
- OpticalDesignofCFX96Real-TimePCRDetectionSystemEliminatestheRequirementofaPassiveReferenceDyeApplicationNote
- Real-TimePCRApplicationsGuide
OnlineResources
- Real-TimePCRDetectionSystemsSelectionChart
- qPCR/Real-TimePCR
- qPCRInstrumentation
Specifications:
| OpticalReactionModule | |
| Modulerequirement | C1000Touchthermalcyclerchassis(catalog#184-1100) |
| Excitation | 6filteredLEDs |
| Detection | 6filteredphotodiodes |
| Rangeofexcitation/emissionwavelengths,nm | 450–730 |
| Sensitivity | Detects1copyoftargetsequenceinhumangenomicDNA |
| Dynamicrange | 10ordersofmagnitude |
