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biospec/SoniBeast - Small Sample Cell Disruptor/1kit/715-12

价格
¥31680.00
货号:715-12
浏览量:127
品牌:biospec
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商品描述

SoniBeast - Small Sample Cell Disruptor

Quicker, Simpler, Less Consumables, Smaller Footprint, Affordable

  • Extremely Fast Cell Lysis: <20 sec
  • Perfect for Small Samples
  • 12 Vial Capacity
  • Simple One Button Operation

The SoniBeast™ is the newest addition to our stable of high energy BeadBeater cell disruptors. Small samples of tissue or cells are completely disrupted in seconds rather than minutes. It processes up to twelve 0.6 ml locking-cap PCR microtubes, each containing 1-100 mg of tissue, or three 2 ml microvials each containing 10-400 mg of tissue. The SoniBeast is designed for wet milling in water-based media. Common applications are the isolation of intracellular protein and nucleic acids.

A pack of polypropylene 0.6 ml locking cap PCR tubes is included with the SoniBeast. Additional Vials should be ordered separately. Beads size and composition vary with the kind of sample being disrupted. For guidance on choosing beads see Lysis Beads or call us at 918-336-3363 for advice.

If you plan to power the SoniBeast with 220-240 Volt AC rather than 110-120 Volt AC please select product number "715-12-EUR".

A hand-held, cordless, smaller capacity version of the Sonibeast is available (product number "SBJ319"). Designed for samples taken in the field or other locations distant from a diagnostic laboratory, it is powered by a rechargeable lithium battery and is capable of complete homogenization of plant or animal tissues in either two 2 ml microvials or one 7 ml vial.

Our Price : $0.00

The SoniBeast™* cell disrupter homogenizes both microorganisms and plant and animal tissue using the smashing action of colliding ceramic beads (commonly called beadbeating). It departs from contemporary beadbeater machines by oscillating microtubes at a rate of 20,000 oscilations/min...some 5 to 50 times faster than currently available beadbeater machines!

As expected, cell disruption occurs much faster when beads inside the tubes are moving at extraordinarily high rates.  Disruption is completed in a few seconds rather than minutes.  Faster disruption times favor higher quality and yields of nucleic acids and proteins because all cells in a tissue sample enter into the protection of the extraction media much quicker.  The operation of SoniBeast cell disrupter is simplified... the machine is operated by the push of a single button.  There is no required manual clamping of tubes or vials onto the machine and the physical locations of vials remain unchanged from their starting point, thus making the SoniBeast the first high-efficiency bead-mill cell disrupter available for robotic systems.

The SoniBeast's tray capacity is one to twelve 0.6 ml locking-cap PCR microtubes, each containing up to 100 mg of sample.  Closer to the axis of the machine is an inner ring having three positions holding larger 1.5 or 2 ml microvials capable of processing up to 400 mg of tissue. Unlike the outer ring of 0.6 ml microvials, samples in these larger microvials will be lysed under the milder grinding conditions typical of earlier beadbeater protocols.

*Patent pending

  • Processes one to twelve 0.6 ml PCR tubes each holding 1-100 mg (wet weight) of tissue or cells at ultra-high shaking energy (typical disruption time 5-20 seconds) or one to three 2 ml screw-cap microvials each holding 10-400 mg (wet weight) tissue or cells at conventional beadbeater shaking energy (typical disruption time 2 minutes).
  • Motor Specifications: 110-120 V~AC, 1.6A, 21,000 rpm
  • Manual pulse or timed operation (0.5, 2, 5, 10, 20, 40, and 60 sec with auto-reset). Activate with the push of a single button.
  • Footprint: 5" wide x 16" deep.  Seven inches high. Weight 11 pounds
  • Two year Total Warranty.  Motor replacement is anticipated with this very high energy machine - especially a with high level of usage.  The SoniBeast is specifically disigned to facilitate fast, easy, on-site change-out.

General Protocol. One to twelve 0.6 ml microtubes are sequentially loaded with beads, tissue sample and extraction media.  After capping, they are inserted into the red silicone rubber grommets in the disc-shaped tube holder.  Unlike all other beadbeaters, inserted tubes do not need to be clamped down..  After selecting a run time (typically 5-20 seconds) the SoniBeast is activated with the touch of a single button.  Seconds later the SoniBeast turns off, the beads fall by gravity to the bottom of the vial, and the lysed tissue sample(s) is ready to transfer by micropipette for subsequent purification and/or analysis.

See https://biospec.com/instructions/sonibeast for detailed operating instructions.

https://biospec.com/product/sonibeast-jr

biospec的Beadbeater(珠磨机)细胞破碎机珠磨(Beadbeating)是用于破坏组织细胞和微生物以回收和分析细胞内蛋白质,核酸和细胞器的首选常规实验室方法。它快速,高效且非常生物安全。有时建议使用附件来控制细胞破裂期间的样品加热。最早于1979年引入研发实验室的BioSpec Products生产五种型号的Beadbeater。每个都针对不同的样品大小和样品瓶容量进行了优化。根据所选择的模型,一个下午可以轻松处理20到2000个样品。根据磁珠的机械搅拌方式,将模型分为三类:从每个Beadbeater模型获得的最终结果非常相似。的NEW SoniBeast ™珠磨机使用专利申请中的珠混合技术。与市场上传统的珠磨机类型的细胞破碎仪(包括我们的MiniBeadbeaters)相比,它提供了更快的细胞破碎能力(最高10倍),并且旨在处理较小的样品。SoniBeast的容量为十二个0.6 ml微型管或三个2 ml微型瓶。 Mini-Beadbeater ™细胞破坏器有四种型号。全部使用标准的3/4“-1”,来回(从上到下)摇动以及“ figure-8”模式,并且经过精心设计,可以在组内提供协议兼容。新的MBB1-Plus一次可处理一个2 ml的小瓶。的MBB-16和MBB-24细胞破坏剂具有多瓶的能力和可以同时使用2位和7毫升microvials。高通量MBB-96可处理一个或两个深孔微孔板,可容纳多达45个微瓶的样品架或可容纳两个50 ml螺帽管的样品架。这组干扰物的可接受样本量为1毫克至1.5克(湿重)组织或堆积细胞。  Mini-Beadbeater的摇动能量等于或超过所有竞争品牌的摇动式珠磨机。钍ë 珠磨器 ™是一个更大容量的珠磨器即可以在进程微生物或单细胞植物或动物细胞的10-80克(湿重)。快速旋转的铁氟龙转子可以摇晃珠子,而不是摇晃运动,它可以在密封的腔室内搅动珠子。它通常用于破坏细胞悬液,例如酵母或细菌,以产生天然或表达的细胞内蛋白。与实验室中使用的其他大多数细胞破坏方法不同,“ Beadbeater方法”可轻松扩展至中试工厂和全面生产水平。