Product Description

Recombinant HEK293 cell line expressing human TRPC3 (transient receptor potential cation channel, subfamily C, member 3, accession number NM_003305). Format Each vial contains 1 X 10^6 cells in 1 ml of 10% DMSO. Mycoplasma Testing The cell line has been screened using the PCR-based VenorGeM Mycoplasma Detection kit (Sigma-Aldrich) to confirm the absence of Mycoplasma species. Introduction TRPC3 channel belongs to the transient receptor potential channel (TRP) superfamily which is divided into seven main subfamilies. TRP channels share a similar architecture of six-transmembrane domains and function as tetramers. Most channels are permeable to calcium and monovalent cations (Vassort et al, 2009). Specifically, TRPC3 channel belongs to a canonical TRP (TRPC) subfamily that is involved in calcium entry. TRPC3 is activated through phospholipase C-linked receptors. When expressed in HEK293 cells, TRPC3 behaves as a receptor activated channel with constitutive activity that cannot be further increased by store depletion (Trebak et al, 2002).

References

Albert A.P., Saleh S.N., and Large W.A. (2009) Identification of canonical transient receptor potential (TRPC) channel proteins in native vascular smooth muscle cells. Current medicinal chemistry 16: 1158-1165.

Lievremont J., Bird G.J., and Putney, Jr. J.W. (2004) Canonical transient receptor potential TRPC7 can function as both a receptor- and store-operated channel in HEK-293 cells. Am J Physiol Cell Physiol 287: C1709-C1716.

Trebak M., Bird G.J., McKay R.R., Putney, Jr. J.W. (2002) Comparison of human TRPC3 channels in receptor-activated and store-operated modes. Journal of Biological Chemistry 277: 21617-21623.

Vassort G. and Alvarez J. (2009) Transient receptor potential: a large family of new channels of which several are involved in cardiac arrhythmia. Can. J. Physiol. Pharmacol. 87: 100-107.