Description: HumanHistone3(HIST1H3E),GenBank Accession No. NM_003532, a.a. 2-137(end) withN-terminalHis-tagMW=15.4kDa, expressed in an E. coli expression system. Methylation Assay:Histone H3 was coated onto a 96-well plate, and methylationby G9a wascarriedoutat roomtemperaturefor60min.Methylated Histone H3 wasdetectedusing Chemiluminescence assay. Application:Usefulforthestudyofenzymekinetics, screeninginhibitors,andselectivity profiling. Formulated in: 25mMTris-HCl,pH8.0, 130 mM NaCl, 10% glycerol, and 1 mM DTT.Stability: >6 months at –80°C