Product information "Phalloidin-iFluor(TM) 488 Conjugate"
This green fluorescent phalloidin conjugate (equivalent to Alexa Fluor(R) 488-labeled phalloidin) selectively binds to F-actins with much higher photostability than the fluorescein-phalloidin conjugates. Used at nanomolar concentrations, phalloidin derivatives are convenient probes for labeling, identifying and quantitating F-actins in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments. Phalloidin binds to actin filaments much more tightly than to actin monomers, leading to a decrease in the rate constant for the dissociation of actin subunits from filament ends, essentially stabilizing actin filaments through the prevention of filament depolymerization. Moreover, phalloidin is found to inhibit the ATP hydrolysis activity of F-actin. Phalloidin functions differently at various concentrations in cells. When introduced into the cytoplasm at low concentrations, phalloidin recruits the less polymerized forms of cytoplasmic actin as well as filamin into stable "islands" of aggregated actin polymers, yet it does not interfere with stress fibers, i.e. thick bundles of microfilaments. The property of phalloidin is a useful tool for investigating the distribution of F-actin in cells by labeling phalloidin with fluorescent analogs and using them to stain actin filaments for light microscopy. Fluorescent derivatives of phalloidin have turned out to be enormously useful in localizing actin filaments in living or fixed cells as well as for visualizing individual actin filaments in vitro. Fluorescent phalloidin derivatives have been used as an important tool in the study of actin networks at high resolution. AAT Bioquest offers a variety of fluorescent phalloidin derivatives with different colors for multicolor imaging applications.
Supplier: | AAT Bioquest |
Supplier-Nr: | 23115 |
Properties
Application: | Labeling |
MW: | 1,9 kD |
Handling & Safety
Storage: | -20°C |
Shipping: | +20°C (International: +20°C) |
Signal Word: | Danger |
GHS Hazard Pictograms: | |
H Phrases: | H301, H311, H331 |
P Phrases: | P261, P280, P302, P312, P361 |
Caution Our products are for laboratory research use only: Not for administration to humans!
美国生物学(USBiologic)位于美国马萨诸塞州的Swampscott。它的生产能力包括抗生素,抗体,琼脂糖,白蛋白,氨基酸,生化试剂,生物制剂,细胞因子,酶,培养基,分子生物学试剂盒,肽和血清。所有产品均提供经过认证的测试结果。USBio提供了多种抗体。