The Blyscan™ Glycosaminoglycan Assay is a quantitative dye-binding method for the analysis of sulfated proteoglycans and glycosaminoglycans, (sGAG).

Test material includes:

• Cell culture derived material
• Soluble sGAGs from fluids, such as synovial fluid, urine.
• Cartilage
• Connective tissue / tumours

Soluble sGAGs may be assayed directly, solid materials will require a preliminary papain extraction.

The assay can be used to measure the total sGAG content and can also be adopted to determine the O- and N-sulfated glycosaminoglycan ratio within test samples.

The dye label used in the assay is 1,9-dimethylmethylene blue and the dye is employed under conditions that provide a specific label for the sulfated polysaccharide component of proteoglycans or the protein free sulfated glycosaminoglycan chains.

Please note:

The assay is not suitable for small sulfated disaccharide fragments or for the samples containing alginates, as these contain uronic acid.

Biocolor弹性蛋白测定– Fastin™弹性蛋白Fastin™弹性蛋白测定是一种定量染料结合方法，用于分析 释放到组织培养基中并从生物材料中提取的弹性蛋白。所使用的染料标记是5,10,15,20-四苯基-21H，23H-卟啉四磺酸盐（TPPS）。弹性蛋白形式可以通过Fastin分析测量为α-弹性蛋白：可溶性原弹性蛋白；促弹性蛋白不溶弹性蛋白（在溶解为弹性蛋白多肽[α-弹性蛋白，κ-弹性蛋白]之后）染料试剂与哺乳动物弹性蛋白中的“基本”和“非极性”氨基酸序列结合。为了分析可溶性弹性蛋白，样品应不含任何颗粒物质（细胞碎片，不溶性细胞外基质物质）。其他可溶性蛋白质或复杂碳酸钙的存在不会干扰Fastin检测。