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蚂蚁淘在线 / 品牌中心 / BioBasic / BioBasic/Extraction Buffer for 2D Gel with Diluent, 50ml Urea, Thiourea, CHAPS and SB 3-10/50ml/PL041
商品描述
Product Description:Extraction Buffer for 2D Gel with Diluent, 50ml Urea, Thiourea, CHAPS and SB 3-10: 2D buffers must solubilize proteins effectively without disturbing the native charge on the proteins. Urea, a common chaotrope, is widely used for solubilization and denaturation of proteins. One of the disadvantages of using urea is carbamylation. Urea in water exists in equilibrium with ammonium cyanate, of whose level increases with increasing temperature and pH. Cyanate reacts with α-amino and ε-amino groups of proteins and induces a change in the isoelectric point. This leads to artifacts in results and therefore carbamylation must be avoided. One way to minimize the risk of carbamylation is to prepare the urea based reagents fresh before each use. Bio Basic Inc. has developed a series of dry urea based pre-mixed and ready-to-use solubilization buffers. Simply add an appropriate volume of the supplied diluent buffer to the dry powder and then use directly to solubilize proteins, saving time and improving the quality of IEF/2D gel electrophoresis. 2D Sample Extraction Buffers are also designed to be used as rehydration buffers for IPG strips. 2D Sample Extraction Buffers are experimentally optimized with proprietary technologies and critical buffering and stabilizing agents, including urea, thiourea, Nonidet® P-40, CHAPS, and sulfobetaines. Our 2D Sample Extraction Buffers are designed to produce optimal protein extraction and significantly improved spot resolution for 2D gel analysis. A range of 2D Sample Extraction Buffers have been developed and depending on the nature of the samples, one or more of the buffers suitable for your applications can be ordered. PL037 is suitable for most applications, however for stronger solubilization effects, we recommend PL038-PL042.
Total Product Size:50ml
Individual Container Size:50ml
Number of Containers:1
Refrigeration Requirements:Freezer
Shipping Conditions:ICE
UNSPSC Code:41105323
UNSPSC Category:Electrophoresis Buffers
Bio Basic（又称“ Bio Basic Group”和“ Bio Basic Canada”）于2004年在加拿大多伦多成立，最初是从其制造的生化试剂的分销开始的。继成功之后，Bio Basic从2008年开始迅速扩展其制造设施，包括寡核苷酸，基因和肽合成，DNA测序以及实验室塑料制品和分子生物学试剂盒等系列。从2009年起，Bio Basic通过引入蛋白质纯化和抗体合成来进一步扩展其服务范围，以完善其现有产品和服务。 
