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蚂蚁淘在线 / 品牌中心 / BioBasic / BioBasic/5X All-In-One RT MasterMix (with Cell Lysis Kit)/100prep/HRT100-20L
商品描述
Product Description:5X All-In-One RT MasterMix (with Cell Lysis Kit): BBI 5X All-In-One RT MasterMix (with ExCellenCT Lysis Kit) offers a quick, simple and robust method to prepare template for fi rst-strand cDNA synthesis directly from cultured cells, enabling reverse transcription of lysates from 10-105 cultured cells without time-consuming and hazardous-chemicals-involved RNA extraction and purification steps. The kit includes reagents for cell lysis as well as gDNA removal. The presence of contaminating genomic DNA (gDNA) in RNA preparations is often a significant problem for downstream applications, leading to false-positive signals and misinterpretation of gene expression levels. The lysis procedure simultaneously eliminates genomic DNA effectively in 12 minutes, without compromising RNA quality, and therefore ensures consistent, reproducible, and accurate results with 10-105 cells.The lysate can then be directly reverse-transcribed into cDNA using the 5X All-In-One RT MasterMix. The 5X All-In-One RT MasterMix is a ready-to-use formulation of all the reagents necessary for fi rst-strand cDNA synthesis, including proprietary ReverseTranscriptase, Ribonuclease Inhibitor, dNTPs and a finelybalanced ratio of Oligo (dT)s and Random Primers. Coupled together, this complete system provides the ultimate convenience in generating high-quality cDNA directly from 10-105 cultured cells, suitable for a wide range of downstream applications.
Total Product Size:100prep
Individual Container Size:100prep
Number of Containers:1
Refrigeration Requirements:Freezer
Shipping Conditions:ICE
UNSPSC Code:41106313
UNSPSC Category:RT-PCR Kits
Bio Basic（又称“ Bio Basic Group”和“ Bio Basic Canada”）于2004年在加拿大多伦多成立，最初是从其制造的生化试剂的分销开始的。继成功之后，Bio Basic从2008年开始迅速扩展其制造设施，包括寡核苷酸，基因和肽合成，DNA测序以及实验室塑料制品和分子生物学试剂盒等系列。从2009年起，Bio Basic通过引入蛋白质纯化和抗体合成来进一步扩展其服务范围，以完善其现有产品和服务。 
