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蚂蚁淘在线 / 品牌中心 / BioAssay Systems / BioAssay Systems/EnzyFluo™ ERK Phosphorylation Assay Kit/100 tests/EERK-100
商品描述
EnzyFluo™ ERK Phosphorylation Assay Kit
- Product Overview
- Product FAQ
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- Assay Service
ProtocolSDS
Application
- For quantitative fluorescent immunoenzymatic assay of ERK1/2 phosphorylation status in cultured cells.
Key Features
- Safe. Non-radioactive assay.
- Simple and convenient. Total and pERK can be measured in the same sample.
Method
- FL360/450nm, 530/585nm
Samples
- Cells
Species
- Human, mouse, rat.
Size
- 100 tests
Shelf Life
- 6 months
More Details
- The mitogen-activated protein kinase (MAPK/ERK) pathway plays a key role in cell proliferation, differentiation and migration. Stimulation by mitogens eventually leads to phosphorylation of ERK1 (T202/Y204) and ERK2 (T185/Y187). The MAPK/ERK cascade presents many interesting drug targets for the development of cancer therapies. BioAssay Systems cell-based ELISA measures dually phosphorylated ERK1/2 in whole cells and normalizes the signal to the total protein content. This simple and efficient assay eliminates the need for cell lysate preparation and can be used to study kinase signaling and the effects of kinase inhibitors on cells. In this assay, cells are grown in 96-well plates and treated with ligands or drugs. Cells are then fixed and permeabilized in the wells. ERK1/2 phosphorylation (pERK) using a fluorescent ELISA followed by total protein measurement in each well.
For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.
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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.Simply send us your samples:- Fast turnaround - Quality data - Low costPlease email or call 1-510-782-9988 x 2 to request assay service.
