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4000-520-616 / 18915418616
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0512-67156496
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商品描述
EnzyChrom™ NAD/NADH Assay Kit
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ProtocolSDS
Application
- For sensitive determination of NAD and NADH and evaluation of drug effects on NAD/NADH metabolism.
Key Features
- Sensitive and accurate. Detection limit of 0.05 μM and linearity up to 10 μM NAD+/NADH in 96-well plate assay.
- Convenient. The procedure involves adding a single working reagent, and reading the optical density at time zero and 15 min at room temperature.
- High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.
Method
- OD565nm
Samples
- Cell or tissue extracts
Species
- All
Size
- 100 tests
Detection Limit
- 0.05 μM
Shelf Life
- 6 months
More Details
- Pyridine nucleotides play an important role in metabolism and, thus, there is continual interest in monitoring their concentration levels. Quantitative determination of NAD+/NADH has applications in research pertaining to energy transformation and redox state of cells or tissue. Simple, direct and automation-ready procedures for measuring NAD+/NADH concentration are very desirable. BioAssay Systems EnzyChrom™ NAD+/NADH assay kit is based on a lactate dehydrogenase cycling reaction, in which the formed NADH reduces a formazan (MTT) reagent. The intensity of the reduced product color, measured at 565 nm, is proportional to the NAD+/NADH concentration in the sample. This assay is highly specific for NAD+/NADH and with minimal interference (<1%) by="">+/NADPH. Our assay is a convenient method to measure NAD, NADH and their ratio.
When tissue is used, should it be freshly obtained? Or is -80°C storage ok?
If direct sample processing is not possible, we recommend snap freezing tissue samples in liquid nitrogen and keeping them either in liquid nitrogen or at -80°C until further processing.For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.Altamimi, T. R. (2018). Integrated Regulation of Cardiac Fatty Acid and Glucose Oxidation. Assay: NAD/NADH in mice heart tissue.Paul, S., Gangwar, A., Bhargava, K., & Ahmad, Y. (2018). STAT3-RXR-Nrf2 activates systemic redox and energy homeostasis upon steep decline in pO2 gradient. Redox biology, 14, 423-438. Assay: NAD/NADH in sprague dewley rats tissue. Wang, W., Hu, Y., Wang, X., Wang, Q., & Deng, H. (2018). ROS-Mediated 15-Hydroxyprostaglandin Dehydrogenase Degradation via Cysteine Oxidation Promotes NAD+-Mediated Epithelial-Mesenchymal Transition. Cell chemical biology, 25(3), 255-261. Assay: NAD/NADH in mice tissue. Wang, Z., Jiang, M., Guo, X., Liu, Z., & He, X. (2018). Reconstruction of metabolic module with improved promoter strength increases the productivity of 2-phenylethanol in Saccharomyces cerevisiae. Microbial cell factories, 17(1), 60. Assay: NAD/NADH in yeast cells. He, X., Wu, C., Cui, Y., Zhu, H., Gao, Z., Li, B. & Zhao, B. (2017). The aldehyde group of gossypol induces mitochondrial apoptosis via ROS-SIRT1-p53-PUMA pathway in male germline stem cell. Oncotarget, 8(59), 100128. Assay: NAD/NADH in cotton cells. Qiao, A., Jin, X., Pang, J., Moskophidis, D., & Mivechi, N. F. (2017). The transcriptional regulator of the chaperone response HSF1 controls hepatic bioenergetics and protein homeostasis. J Cell Biol, 216(3), 723-741. Assay: NAD/NADH in mice liver tissue. Bae, S. J., Kim, S., & Hahn, J. S. (2016). Efficient production of acetoin in Saccharomyces cerevisiae by disruption of 2, 3-butanediol dehydrogenase and expression of NADH oxidase. Scientific reports, 6, 27667. Assay: NAD/NADH in yeast cells. Bai P et al (2011). PARP-1 inhibition increases mitochondrial metabolism through SIRT1 activation. Cell Metab. 13(4):461-8. Assay: NAD/NADH in mouse cells. Koo BS et al (2010). Improvement of coenzyme Q(10) production by increasing the NADH/NAD(+) ratio in Agrobacterium tumefaciens. Biosci Biotechnol Biochem.74(4):895-8. Assay: NAD/NADH in yeast Agrobacterium tumefaciens. Lee M et al (2010). Depletion of GSH in glial cells induces neurotoxicity: relevance to aging and degenerative neurological diseases. FASEB J. 24(7):2533-45. Assay: NAD/NADH in human cell. Hsu CP et al (2009). Nicotinamide phosphoribosyltransferase regulates cell survival through NAD+ synthesis in cardiac myocytes. Circ Res. 105(5):481-91. Assay: NAD/NADH in mouse heart cardiac myocytes. Tseng HC et al (2009). Metabolic engineering of Escherichia coli for enhanced production of (R)- and (S)-3-hydroxybutyrate. Appl Environ Microbiol. 75(10):3137-45. Assay: NAD/NADH in bacteria E.coli. Clem B,et al (2008). Small-molecule inhibition of 6-phosphofructo-2-kinase activity suppresses glycolytic flux and tumor growth. Mol Cancer Ther. 7(1):110-20. Assay: NAD/NADH in human epithelial cell. Greenall A et al (2008). A genome wide analysis of the response to uncapped telomeres in budding yeast reveals a novel role for the NAD+ biosynthetic gene BNA2 in chromosome end protection. Genome Biol. 9(10):R146. Assay: NAD/NADH in yeast cell. Kim Y, et al (2008). Dihydrolipoamide dehydrogenase mutation alters the NADH sensitivity of pyruvate dehydrogenase complex of Escherichia coli K-12. J Bacteriol. 190(11):3851-8. Assay: NAD/NADH in bacterial E. coli. Olesen UH, et al (2008). Anticancer agent CHS-828 inhibits cellular synthesis of NAD. Biochem Biophys Res Commun. 367(4):799-804. Assay: NAD/NADH in human cell. Song HK, et al (2008). Visfatin: a new player in mesangial cell physiology and diabetic nephropathy. Am J Physiol Renal Physiol. 295(5):F1485-94. Assay: NAD/NADH in human mesangial cells. Thornburg JM et al (2008). Targeting aspartate aminotransferase in breast cancer. Breast Cancer Res. 10(5):R84. Assay: NAD/NADH in human breast adenocacinoma cell.To find more recent publications, pleaseclick here.
If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.Simply send us your samples:- Fast turnaround - Quality data - Low costPlease email or call 1-510-782-9988 x 2 to request assay service.
