BioAssay Systems/QuantiFluo™ ALP Reagent/600 tests/QFALP-6mL

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¥2100.00
货号:QFALP-6mL
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商品描述

QuantiFluo™ ALP Reagent

QuantiFluo™ ALP Reagent Catalog No: QFALP-6mL
Price: $105 Qty:
For orders of 10 or more kits, please call +1-510-782-9988x1 oremail us for best pricing and/or bulk order. Shipping: RT Shipment: Fedex ServiceDelivery: 1-2 days (US), 3-6 days (Intl) Storage: -20°C
ALP Detection Reagent (QFALP-6mL)
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ProtocolSDS

Application

  • Stabilized 4-methylumbelliferyl phosphate (MUP) liquid substrate reagent for detecting alkaline phosphatase ALP, e.g. in ELISA. This single reagent produces a highly fluorescent product (360/450 nm) when dephosphorylated by ALP. The fluorescent product can also be visualized under a UV light.

Key Features

  • Stable single reagent.
  • Highly sensitive fluorescence detection.
  • Can be visualized under a UV light.

Method

  • FL360/450nm

Samples

  • Biological

Species

  • All

Size

  • 600 tests

Shelf Life

  • 2 years

More Details

  • QuantiFluo™ ALP Reagent is a stable 4-methylumbelliferyl phosphate (MUP) liquid substrate reagent formulated for detecting alkaline phosphatase ALP, e.g. in ELISA. This single reagent produces a highly fluorescent product (λex/em = 360/450 nm) when dephosphorylated by ALP. The fluorescent product can also be visualized under a UV light.
For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.
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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.Simply send us your samples:- Fast turnaround - Quality data - Low costPlease email or call 1-510-782-9988 x 2 to request assay service.
BioAssay Systems的EnzyFluo™ERK磷酸化检测试剂盒的有丝分裂原活化蛋白激酶(MAPK / ERK)途径在细胞增殖,分化和迁移中起关键作用。有丝分裂原刺激最终导致ERK1(T202 / Y204)和ERK2(T185 / Y187)磷酸化。MAPK / ERK级联为癌症治疗的发展提出了许多有趣的药物靶标。BioAssay Systems基于细胞的ELISA可测量全细胞中双重磷酸化的ERK1 / 2,并将信号归一化至总蛋白质含量。这种简单有效的测定方法消除了细胞裂解液制备的需要,可用于研究激酶信号传导和激酶抑制剂对细胞的作用。在该测定中,细胞在96孔板中生长,并用配体或药物处理。然后将细胞固定并在孔中透化。使用荧光ELISA进行ERK1 / 2磷酸化(pERK),然后在每个孔中测量总蛋白。