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苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
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0512-67156496
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info@ebiomall.com
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https://www.ebiomall.com
商品描述
QuantiFluo™ DNA Assay Kit
- Product Overview
- Product FAQ
- Product Citations
- Assay Service
ProtocolSDS
Application
- For quantitative determination of DNA.
Key Features
- Sensitive and accurate. Linear detection range 2 ng to 40 ng (100 - 2,000 ng/mL) calf thymus DNA in 96-well plate assay.
- Simple and high-throughput. The "mix-and-read" procedure involves addition of a single working reagent and reading the fluorescence intensity. Can be readily automated as a high-throughput assay for thousands of samples per day.
- Low interference. RNA, salt (up to 3M NaCl), detergent (< 0.01%="" sds)="" and="" common="" dna="" extraction="" buffer="" do="" not="" interfere="" in="" the="">
Method
- FL340/450nm
Samples
- Plasmid DNA, genomic DNA, cDNA, DNA following polymerase chain reaction, and DNA extracted from gel and other matrices
Species
- All
Size
- 250 tests
Detection Limit
- 100 ng/mL
Shelf Life
- 12 months
More Details
- DNA quantitation is a common practice in molecular biology. Very often DNA is available in minute quantities and the traditional UV 260 nm absorbance method requires microgram quantities for reliable results. Accurate determination of DNA concentration, especially when DNA is present at low concentrations, is crucial for reproducible results in sequencing, cloning, transfection and DNA labeling. Simple, direct and automation-ready procedures for measuring DNA concentration are very desirable. BioAssay Systems QuantiFluo™ DNA assay kit is designed to accurately measure nanogram quantities of plasmid DNA, cDNA, DNA following polymerase chain reaction and DNA eluted from gels. The improved method utilizes Hoechst dye that binds specifically with double-stranded DNA. The fluorescence intensity, measured at λex/em = 350/450nm, is directly proportional to the DNA concentration in the sample. The optimized formulation substantially reduces interference by substances in the raw samples.
Can you guarrantee, that your kit enables an accurate estimation of cDNA concentration in RT reaction mixture, where high excess of total RNA is present? Is it a SYBR Green based method?
We are using Hoechst dye based DNA assay. It is very specific for DNA and not RNA. However, as we do not know the RNA content in your sample, we can not judge if it would meet your needs.For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.Mayer-Wagner S, et al (2011). Effects of low frequency electromagnetic fields on the chondrogenic differentiation of human mesenchymal stem cells. Bioelectromagnetics. 32(4):283-90. Assay: DNA in human stem cell. Waheed MM et al (2011). Some Biochemical Characteristics and Preservation of Epididymal Camel Spermatozoa (Camelus dromedarius). Theriogenology 2011 Jul 13. Assay: DNA in human cell line.Labuzek K, et al (2010). Metformin has adenosine-monophosphate activated protein kinase (AMPK)-independent effects on LPS-stimulated rat primary microglial cultures. Pharmacol Rep. 62(5):827-48. Assay: DNA in rat microglial cells. Labuzek K, et al (2010). Ambivalent effects of compound C (dorsomorphin) on inflammatory response in LPS-stimulated rat primary microglial cultures. Naunyn Schmiedebergs Arch Pharmacol. 381(1):41-57. Assay: DNA in rat primary glial culture. Mayer-Wagner S et al. (2010). Membrane-based cultures generate scaffold-free neocartilage in vitro: influence of growth factors. Tissue Eng Part A. 16(2):513-21. Assay: DNA in human cell. Bhavnani B.R. et al. (2008). Structure activity relationships and differential interactions and functional activity of various equine estrogens mediated via estrogen receptors (ERs) ERalpha and ERbeta. Endocrinology. 149(10):4857-70. Assay: DNA in human cell line. Lai, M et al (2007). Development of an In-Vitro Screening Method to Determine Enzymatic Release rates from a Homologous Series of Novel SN-38 Conjugates. Sonus Pharmaceuticals. 2007 AAPS Annual Meeting & Exposition. Assay: DNA in human cells, stem cells. To find more recent publications, pleaseclick here.
If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.Simply send us your samples:- Fast turnaround - Quality data - Low costPlease email or call 1-510-782-9988 x 2 to request assay service.
