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蚂蚁淘在线 / 品牌中心 / BioAssay Systems / BioAssay Systems/QuantiChrom™ Glucose-6-Phosphate Dehydrogenase Kit/100 tests/DGPDH-100
商品描述
QuantiChrom™ Glucose-6-Phosphate Dehydrogenase Kit
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- Product FAQ
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- Assay Service
ProtocolSDS
Application
- For quantitative colorimetric determination of Glucose-6-Phosphate concentration and evaluation of drug effects on its metabolism.
Key Features
- Fast and sensitive. Linear detection range (20 μL sample): 0.2 to 100 U/L for 15 min reaction.
- Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Method
- OD460nm
Samples
- Plasma, serum, tissue and culture media, etc
Species
- All
Size
- 100 tests
Detection Limit
- 0.2 U/L
Shelf Life
- 6 months
More Details
- GLUCOSE-6-PHOSPHATE DEHYDROGENASE (G6PDH) is a cytosolic enzyme in the pentose phosphate pathway which supplies reducing energy to cells by maintaining the level of the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH). G6PDH reduces nicotinamide adenine dinucleotide phosphate (NADP) to NADPH while oxidizing glucose-6-phosphate (G6P). Humans with a genetic deficiency of G6PDH are predisposed to non-immune hemolytic anemia. BioAssay Systems' non-radioactive, colorimetric G6PDH assay is based on the reduction of the tetrazolium salt MTT in a NADPH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is proportional to the enzyme activity.
For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.
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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.Simply send us your samples:- Fast turnaround - Quality data - Low costPlease email or call 1-510-782-9988 x 2 to request assay service.
