EnzyChrom™ Aspartate Transaminase Assay Kit

I am not quite clear about the factor 388 used in the calculation. How is path length correction for 220 µl of sample volume in the 96 well done? Is the concentration of the NADH used 10 mM?

The concentration of the NADH solution is 10 mM. The concentration of NADH in the final reaction volume (V = 220 μL) is calculated by accounting for the dilution steps when preparing the working reagent, i.e. 4 μL/206μL in the first step and 200 mL/220 mL in the second step. This is the final initial concentration of NADH in the reaction.To calculate the activity of the sample, i.e. µmoles NADH consumed / (L x min), one has to divide by 5 min incubation time and multiply by the dilution factor of the sample which is 11.Multiplying all these constant values together equals 388.There is no need for a path length correction, because the sample and the standard wells contain the same volume.For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.

Li, Yajuan, et al (2019). PTEN-induced partial epithelial-mesenchymal transition drives diabetic kidney disease. The Journal of clinical investigation 129.3. Assay: Aspartate transaminase in mice serum. Hay, Charles E., et al (2018). Development and testing of AAV-delivered single-chain variable fragments for the treatment of methamphetamine abuse. PloS one 13.6: e0200060. Assay: Aspartate transaminase in mice serum. Kamerkar, Sushrut, et al (2017). Exosomes facilitate therapeutic targeting of oncogenic KRAS in pancreatic cancer. Nature 546.7659: 498. Assay: Aspartate transaminase in mice plasma. Kim, Soo Hyun, et al (2017). Ezetimibe ameliorates steatohepatitis via AMP activated protein kinase-TFEB-mediated activation of autophagy and NLRP3 inflammasome inhibition. Autophagy 13.10: 1767-1781. Assay: Aspartate transaminase in mice serum. Sharabi, K et al (2017). Selective chemical inhibition of PGC-1alpha gluconeogenic activity ameliorates type 2 diabetes. Cell 169.1: 148-160. Assay: Aspartate transaminase in mice serum. Wardah, J. Rahmahani, and T. Sopandi (2017). Effect of Phyllanthus buxifolius Leaf as a Feed Supplement on Liver Function and Haematological Response of Quail (Coturnix coturnix japonica) Challenged with Infectious Newcastle Disease Virus. International Journal of Poultry Science 16: 354-363. Assay: Aspartate transaminase in quail serum.Weis, Sebastian, et al (2017). Metabolic adaptation establishes disease tolerance to sepsis. Cell 169.7: 1263-1275. Assay: Aspartate transaminase in mice plasma. Seemann, Semjon, and Amelie Lupp (2016). Administration of AMD3100 in endotoxemia is associated with pro-inflammatory, pro-oxidative, and pro-apoptotic effects in vivo. Journal of biomedical science 23.1: 68. Assay: Aspartate transaminase in mouse serum. Ballester-Lozano GF et al. (2015). Comprehensive biometric, biochemical and histopathological assessment of nutrient deficiencies in gilthead sea bream fed semi-purified diets. Br J Nutr. 114(5):713-26. Assay: aspartate aminotransferase enzyme activity in fish. Laura, BP et al. (2015). Lasting effects of butyrate and low FM/FO diets on growth performance, blood haematology/biochemistry and molecular growth-related markers in gilthead sea bream (Sparus aurata). Aquaculture 454: 8-18. Assay: Aspartate transaminase in fish plasma.Seemann, S and A Lupp (2015). Administration of a CXCL12 Analog in Endotoxemia Is Associated with Anti-Inflammatory, Anti-Oxidative and Cytoprotective Effects In Vivo. PLoS One 10(9): e0138389. Assay: Aspartate transaminase in mouse serum. Jiang, S., et al (2014). Effect of perches on liver health of hens. Poultry science, 93(7), 1618-1622. Assay: Aspartate transaminase in hen serum. Vives, V et al (2014). Pharmacological inhibition of Dock5 prevents osteolysis by affecting osteoclast podosome organization while preserving bone formation. Nature Communications 6: 6128. Assay: Aspartate transaminase in mouse serum.To find more recent publications, pleaseclick here.