BioAssay Systems/EnzyChrom™ Aspartate Assay Kit/100 tests/EASP-100

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¥6980.00
货号:EASP-100
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商品描述

EnzyChrom™ Aspartate Assay Kit

EnzyChrom™ Aspartate Assay Kit Catalog No: EASP-100
Price: $349 Qty:
For orders of 10 or more kits, please call +1-510-782-9988x1 oremail us for best pricing and/or bulk order. Shipping: On Ice Shipment: Fedex ServiceDelivery: 1-2 days (US), 3-6 days (Intl) Storage: 4°C
Aspartate Assay Kit
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ProtocolSDS

Application

  • For quantitative determination of aspartate and evaluation of drug effects on aspartate metabolism.

Key Features

  • Sensitive and accurate. Linear detection range: 2 to 400 μM aspartate for colorimetric assays and 1 to 50 μM for fluorimetric assays.

Method

  • OD570nm, or FL530/585nm

Samples

  • Plasma, serum, tissue, culture media etc

Species

  • All

Size

  • 100 tests

Detection Limit

  • OD, FL: 2, 1 μM

Shelf Life

  • 6 months

More Details

  • ASPARTATE , a nonessential amino acid, is a precursor to several other amino acids and is an excitatory neurotransmitter. Aspartate is involved in the urea cycle and gluconeogenesis. BioAssay Systems Aspartate Assay Kit provides a simple, direct and automation-ready procedure for measuring aspartate concentration. Aspartate is converted into pyruvate which is then oxidized with the conversion of the dye into a colored and fluorescent form. The color intensity of the oxidized dye at 570 nm or fluorescence intensity at λex/em = 530/585 nm is directly proportional to the aspartate concentration in the sample.
For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.
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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.Simply send us your samples:- Fast turnaround - Quality data - Low costPlease email or call 1-510-782-9988 x 2 to request assay service.
BioAssay Systems的EnzyFluo™ERK磷酸化检测试剂盒的有丝分裂原活化蛋白激酶(MAPK / ERK)途径在细胞增殖,分化和迁移中起关键作用。有丝分裂原刺激最终导致ERK1(T202 / Y204)和ERK2(T185 / Y187)磷酸化。MAPK / ERK级联为癌症治疗的发展提出了许多有趣的药物靶标。BioAssay Systems基于细胞的ELISA可测量全细胞中双重磷酸化的ERK1 / 2,并将信号归一化至总蛋白质含量。这种简单有效的测定方法消除了细胞裂解液制备的需要,可用于研究激酶信号传导和激酶抑制剂对细胞的作用。在该测定中,细胞在96孔板中生长,并用配体或药物处理。然后将细胞固定并在孔中透化。使用荧光ELISA进行ERK1 / 2磷酸化(pERK),然后在每个孔中测量总蛋白。