BioAssay Systems/QuantiChrom™ GTPase Assay Kit/200 tests/DATG-200

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¥6980.00
货号:DATG-200
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商品描述

QuantiChrom™ GTPase Assay Kit

QuantiChrom™ GTPase Assay Kit Catalog No: DATG-200
Price: $349 Qty:
For orders of 10 or more kits, please call +1-510-782-9988x1 oremail us for best pricing and/or bulk order. Shipping: RT Shipment: Fedex ServiceDelivery: 1-2 days (US), 3-6 days (Intl) Storage: 4°C
GTPase Assay Kit
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ProtocolSDS

Application

  • For quantitative determination of ATPase, GTPase or any enzyme activity that liberated free phosphate and high-throughput screen for their inhibitors.

Key Features

  • High sensitivity: detection of 0.007 U/L ATPase or GTPase activity.
  • Fast and convenient: single reagent, homogeneous "mix-and-measure" assay allows quantitation of enzyme activity within 30 minutes.
  • Robust and amenable to HTS: detection at 620nm greatly reduces potential interference by colored compounds. Z factors of >0.7 are observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems.

Method

  • OD620nm

Samples

  • ATPase and GTPase

Species

  • All

Size

  • 200 tests

Detection Limit

  • 0.007 U/L

Shelf Life

  • 12 months

More Details

  • ATPases and GTPases catalyze the decomposition of ATP or GTP into ADP or GDP and free phosphate ion. These enzymes play key roles in transport, signal transduction, protein biosynthesis and cell differentiation. BioAssay Systems QuantiChrom™ ATPase/GTPase Assay Kit offers a highly sensitive method for determining ATPase/GTPase activities in a microplate format. Its proprietary formulation features a single reagent for accurate determination of enzyme activity in 30 min at room temperature. The improved malachite green reagent forms a stable dark green color with liberated phosphate, which is measured on a plate reader (600 - 660 nm).
For more detailed product information and questions, please feel free to Contact Us. Or for more general information regarding our assays, please refer to our General Questions.
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If you or your labs do not have the equipment or scientists necessary to run this assay, BioAssay Systems can perform the service for you.Simply send us your samples:- Fast turnaround - Quality data - Low costPlease email or call 1-510-782-9988 x 2 to request assay service.
BioAssay Systems的EnzyFluo™ERK磷酸化检测试剂盒的有丝分裂原活化蛋白激酶(MAPK / ERK)途径在细胞增殖,分化和迁移中起关键作用。有丝分裂原刺激最终导致ERK1(T202 / Y204)和ERK2(T185 / Y187)磷酸化。MAPK / ERK级联为癌症治疗的发展提出了许多有趣的药物靶标。BioAssay Systems基于细胞的ELISA可测量全细胞中双重磷酸化的ERK1 / 2,并将信号归一化至总蛋白质含量。这种简单有效的测定方法消除了细胞裂解液制备的需要,可用于研究激酶信号传导和激酶抑制剂对细胞的作用。在该测定中,细胞在96孔板中生长,并用配体或药物处理。然后将细胞固定并在孔中透化。使用荧光ELISA进行ERK1 / 2磷酸化(pERK),然后在每个孔中测量总蛋白。