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Description
The A92-3 antibody reacts specifically with rat IgG2c.It does not react with other Ig isotypes.
Format
- FormatPurified
Suggested Companion Products
Purified Rat IgG2c, κ Isotype Control RUO 0.5mgCat No: 553982
Biotin Mouse Anti-Rat IgG2c A92-1RUO 0.5mgCat No: 553909
Resources & Tools | ||||||
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SpectrumViewer | Download TDS | Regulatory Document Website |
Preparation and Storage
The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.Store undiluted at 4°C.
Product Notices
- Since applications vary, each investigator should titrate the reagent to obtain optimal results.
- Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
- Sodium azide is a reversible inhibitor of oxidative metabolism; therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
- Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purified clone A92-3 is useful as a capture antibody for detection of rat IgG2c in a sandwich ELISA. It is optimal for capture when paired with biotinylated A92-1 mAb (Cat. no. 553909) for detection, and Clone A23-1 as a standard, (Cat. No. 553982). For use in in vivo and in vitro cellular assays, theno azide/low endotoxin format of clone A23-1 is recommended, (Cat. No. 553981).