快报
欢迎光临蚂蚁淘在线！ 请登录 免费注册
商品
品牌
文章
热门搜索：
快报
品牌首页 品牌新闻 品牌简介 产品中心 品牌咨询 联系我们
品牌分类
品牌咨询
联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
蚂蚁淘在线 / 品牌中心 / BD Biosciences / BD Biosciences/CD19 (Leu™-12) PE/20 µL/349209

BD Biosciences/CD19 (Leu™-12) PE/20 µL/349209

价格
面议
货号：349209
浏览量：94
品牌：BD Biosciences
服务
全国联保
正品保证
正规发票
签订合同
商品描述
Product DetailsRecommended AssayReferences

Description

Becton Dickinson Immunocytometry Systems (BDIS) CD19 (Leu™-12) phycoerythrin (PE) is a single-color direct immunofluorescence reagent for the enumeration of B lymphocytes (CD19+) in peripheral blood using fluorescence microscopy or flow cytometers such as the FACSCalibur™, FACSort™, FACScan™, or FACS Analyzer™.

Format
  • FormatPE
  • Excitation SourceBlue 488 nm,Green 532 nm,Yellow/Green 561 nm
  • Excitation Max496nm
  • Emission Max578nm

R-phycoerythrin (PE) is an accessory photosynthetic pigment found in red algae. It exists in vitro as a 240-kDa protein with 23 phycoerythrobilin chromophores per molecule. This makes PE one of the brightest fluorochromes for flow cytometry applications, but its photobleaching properties make it unsuitable for fluorescence microscopy.

Resources & Tools
Spectrum ViewerPanel DesignerSpectrumViewerDownload TDSDownload MSDSCFDA License
Preparation and Storage

1. For in vitro diagnostic use.

2. When stored at 2° to 8°C, the antibody reagent is stable until the expiration date shown on the label. Do not use after the expiration date.

3. The antibody reagent should not be frozen or exposed to direct light during storage or during incubation with cells. Keep the reagent vial dry.

4. Alteration in the appearance of the reagent, such as precipitation or discoloration, indicates instability or deterioration. In such cases, the reagent should not be used.

5. The antibody reagent contains sodium azide as a preservative; however, care should be taken to avoid microbial contamination, which may cause erroneous results.

  1. Brown G, Greaves M. Enumeration of absolute numbers of T and B cells in human blood. Scand J Immunol. 1974;3:161.

  2. Centers for Disease Control. Guidelines for the performance of CD4+ T-cell determination in persons with human immunodeficiency virus infection. MMWR. 1992;41(No. RR-8):1-17.

  3. Centers for Disease Control. Update: Universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens on health-care settings. MMWR. 1988;37(24).

  4. Clark P, Normansell D, Innes D, Hess C. Lymphocyte subsets in normal bone marrow. Blood. 1986;67:1600.

  5. Dwyer J, Finklestein F, Mangi R, Fisher K, Hendler E. Assessment of the adequacy of immunosuppressive therapy using microscopy techniques to study immunologic competence. Transplant Proc. 1975;7:785.

  6. Dörken B, Möller P, Pezzutto A, Schwartz-Albiez R, Moldenhauer G. B-cell antigens: CD19. In: Knapp W, Dörken B, Gilks WR, et al, eds. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:34-36.

  7. Foucar K, Goeken J. Clinical application of immunologic techniques to the diagnosis of lymphoproliferative and immunodeficiency disorders. Lab Med. 1982;13:403-413.

  8. Fröland S, Natvig J, Berdal P Surface-bound immunoglobulin as a marker of B lymphocytes in man. Nature. 1971;234:251-252.

  9. Giorgi J. Lymphocyte subset measurements: significance in clinical medicine. In: Rose N, Friedman H, Fahey J, eds. Manual of Clinical Laboratory Immunology. Washington, DC: American Society for Microbiology; 1986:236-246.

  10. Jackson A, Warner N. Preparation, staining, and analysis by flow cytometry of peripheral blood leukocytes. In: Rose N, Friedman H, Fahey J, eds. Manual of Clinical Laboratory Immunology. Washington, DC: American Society for Microbiology; 1986:226-235.

  11. Kohler G, Milstein C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature. 1975;256:495.

  12. Loken MR, Shah VO, Dattilio KL, Civin CI. Flow cytometric analysis of human bone marrow. II. Normal B-lymphocyte development. Blood. 1987;70:1316-1324.

  13. Meeker TC, Miller RA, Link MP, Bindl J, Warnke R, Levy R. A unique human B lymphocyte antigen defined by a monoclonal antibody. Hybridoma. 1984;3:305-320.

  14. Mishell B, Shiigi S, Henry C, et al. Preparation of mouse cell suspensions. In: Mishell B, Shiigi S, eds. Selected Methods in Cellular Immunology. New York: WH Freeman and Co; 1980:16-17.

  15. Nadler L. B cell/leukemia panel workshop: Summary and comments. In: Reinherz E, Haynes B, Nadler L, ID B, eds. Leukocyte Typing II: Human B Lymphocytes. New York, NY: Springer-Verlag; 1986:3–43.

  16. National Committee for Clinical Laboratory Standards. Clinical Applications of Flow Cytometry: Quality Assurance and Immunophenotyping of Peripheral Blood Lymphocytes (H42-T). 1992

  17. National Committee for Clinical Laboratory Standards. Protection of Laboratory Workers from Infectious Disease Transmitted by Blood, Body Fluids, and Tissue: Tentative Guideline (M29-T2). 1991.

  18. Ryan D, Kossover S, Mitchell S, Frantz C, Hennessy L, Cohen H. Subpopulations of common acute lymphoblastic leukemia antigen-positive lymphoid cells in normal bone marrow identified by hematopoietic differentiation antigens. Blood. 1986;68:417-425.

  19. Tursz T, Brouet J-C, Flandrin G, Danon F, Clauvel J-P, Seligmann M. Clinical and pathologic features of Waldenström"s macroglobulinemia in seven patients with serum monoclonal IgG or IgA. Am J Med. 1977;63:499-502.

  20. Warner N. Membrane immunoglobulins and antigen receptors on B and T lymphocytes. Advances Immunol. 1974;19:67.

BD Biosciences提供了广泛的试剂组合，用于研究细胞凋亡，细胞周期和细胞增殖。已经开发了几种方法来评估各种样品中的细胞凋亡，细胞周期和细胞增殖。我们的试剂可以探索这些过程的细胞特征。此外，BD Biosciences提供了多种工具来协助客户进行实验设置和分析。