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Description
CD1a, clone SK9, is derived from hybridization of mouse NS-1 myeloma cells with spleen cells from CD-1 mice immunized with human thymocytes.
CD1a recognizes a human thymocyte antigen, Mr 49 kilodaltons (kd), associated with 12-kd β2 microglobulin.
Format
- FormatPE
- Excitation SourceBlue 488 nm,Green 532 nm,Yellow/Green 561 nm
- Excitation Max496nm
- Emission Max578nm
R-phycoerythrin (PE) is an accessory photosynthetic pigment found in red algae. It exists in vitro as a 240-kDa protein with 23 phycoerythrobilin chromophores per molecule. This makes PE one of the brightest fluorochromes for flow cytometry applications, but its photobleaching properties make it unsuitable for fluorescence microscopy.
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Preparation and Storage
The monoclonal antibody is supplied as 100 μg purified immunoglobulin in 2.0 mL (50 μg/mL) of phosphate-buffered saline (PBS). The PE conjugate is supplied as 25 μg in 1.0 mL (25 μg/mL) of PBS. PBS contains gelatin and 0.1% sodium azide. Store vials at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.
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- Boumsell L, Amiot M, Raynal B, Gay-Bellile V, Caillou B, Bernard A. Epitope groups of CD1 molecules. In: Reinherz EL, Haynes BF, Nadler LM, Bernstein ID, eds. Leukocyte Typing II: Human T Lymphocytes. Vol 1. New York, NY: Springer-Verlag; 1986:289-302.
- Centers for Disease Control. Perspectives in disease prevention and health promotion update: universal precautions for prevention of transmission of human immunodeficiency virus, hepatitis B virus, and other bloodborne pathogens in health-care settings. MMWR. 1988;37:377-388.
- Dalloul AH, Patry C, Salamero J, Canque B, Grassi F, Schmitt C. Functional and phenotypic analysis of thymic CD34+CD1a– progenitor-derived dendritic cells: predominance of CD1a+ differentiation pathway. J Immunol. 1999;162:5821-5828.
- Foon KA, Gale RP, Todd RF, III. Recent advances in the immunologic classification of leukemia. Semin Hematol. 1986;23:257-283.
- Ito T, Inaba M, Inaba K, et al. A CD1a+/CD11c+ subset of human blood dendritic cells is a direct precursor of Langerhans cells. J Immunol. 1999;163:1409-1419.
- Martin LH, Calabi F, Lefebvre F-A, Bilsland CAG, Milstein C. Structure and expression of the human thymocyte antigens CD1a, CD1b, and CD1c. Proc Natl Acad Sci USA. 1987;84:9189-9193.
- Wood GS, Warner NL, Warnke RA. Anti–Leu-3/T4 antibodies react with cells of monocyte/macrophage and Langerhans lineage. J Immunol. 1983;131:212-216.
- Wood GS, Warner NL, Warnke RA. Anti–Leu-3/T4 antibodies react with cells of monocyte/macrophage and Langerhans lineage. J Immunol. 1983;131:212-216.
