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蚂蚁淘在线 / 品牌中心 / BD Biosciences / BD Biosciences/Stem Cell Control CD34+ Whole Blood Process Control/340991

BD Biosciences/Stem Cell Control CD34+ Whole Blood Process Control/340991

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面议
货号：340991
浏览量：22
品牌：BD Biosciences
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Product DetailsRecommended AssayReferences

Description

The BD™ Stem Cell control is intended as a complete, two-level, process control for immunophenotyping and enumeration of leucocytes by flow cytometry. It is a control for antibody staining, red blood cell (RBC) lysis, instrument setup and performance, and data analysis.

Contents: 1 x 2.0 ml CD34+ Low approx. 10 cells/µl, and 1 x 2.0 ml CD34+ High approx. 35 cells/µl.

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Preparation and Storage

• Store vials upright, tightly capped, at 2°C to 8°C when not in use.

• Unopened vials are stable until the expiration date indicated on each vial and assay sheet.

• Opened vials are stable for 12 thermal cycles (uses) when handled properly. A thermal cycle constitutes performing all steps under Section 5, Instructions for Use, once.

• Avoid unnecessary cycles of warming and cooling. Protect this product from freezing, from temperatures above 30°C, and from prolonged time at room temperature (18°C to 26°C). Follow exactly the steps in Section 5 Instructions For Use.

  1. Clinical Applications of Flow Cytometry: Quality Assurance and Immunophenotyping of Lymphocytes: Approved Guideline. Wayne, PA: Clinical and Laboratory Standards Institute; 1998. NCCLS document H42-A.

  2. Statistical Quality Control for Quantitative Measurements: Principles and Definitions; Approved Guideline. Villanova, Pa: National Committee for Clinical Laboratory Standards; 1999. NCCLS document C-24A2.

  3. Centers for Disease Control. Revised Guidelines for the Performance of CD4+ T-Cell Determinations in Persons with HIV Infection. MMWR. 1994;43(RR-3):1-21.

  4. Gratama JW, Orfao A, Barnett D, et al. Flow cytometric enumeration of CD34+ hematopoietic stem and progenitor cells. Cytometry. 1998;34:128-142.

  5. Marti G, Johnsen H, Sutherland R, Serke S. Letter to the Editor: A convergence of methods for a worldwide standard for CD34+ cell enumeration. J Hematotherapy. 1998;7:105-109.

  6. Stelzer GT, Marti G, Hurley A, McCoy P, Jr, Lovett EJ, Schwartz A. US-Canadian consensus recommendations on the immunophenotypic analysis of hematologic neoplasia by flow cytometry: standardization and validation of laboratory procedures. Cytometry. 1997;30:214-230.

  7. Sutherland DR, Anderson L, Keeney M, Nayar R, Chin-Yee I. The ISHAGE guidelines for CD34+ cell determination by flow cytometry. J Hematotherapy. 1996;5:213-226.

  8. Villanova, PA: National Committee for Clinical Laboratory Standards; Clinical Applications of Flow Cytometry: Immunophenotyping of Leukemic Cells; Approved Guideline. Villanova, PA: Natinal Committee for Clinical Laboratory Standards; 1998. H43-A.

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