快报
欢迎光临蚂蚁淘在线！ 请登录 免费注册
商品
品牌
文章
热门搜索：
快报
品牌首页 品牌新闻 品牌简介 产品中心 品牌咨询 联系我们
品牌分类
品牌咨询
联系方式
公司地址
苏州工业园区生物纳米园A4#216
联系电话
4000-520-616 / 18915418616
传真号码
0512-67156496
电子邮箱
info@ebiomall.com
公司网址
https://www.ebiomall.com
蚂蚁淘在线 / 品牌中心 / BD Biosciences / BD Biosciences/CD3 FITC/CD16+CD56 PE/340042

BD Biosciences/CD3 FITC/CD16+CD56 PE/340042

价格
面议
货号：340042
浏览量：91
品牌：BD Biosciences
服务
全国联保
正品保证
正规发票
签订合同
商品描述
Product DetailsRecommended AssayReferences

Description

Becton Dickinson Immunocytometry Systems (BDIS) Simultest™ CD3/CD16+CD56 (Leu™- 4/11c+19) is a two-color direct immunofluorescence reagent for enumerating percentages of mature human natural killer (NK) lymphocytes in erythrocyte-lysed whole blood (LWB).

Contents

SpecificityCloneFormatIsotypeEntrez Gene ID
CD3SK7 (also known as Leu-4)FITCIgG1, κ916
CD56 (NCAM-1)MY31PEIgG1, κ
CD16 (FcγRIII)B73.1PEIgG1, κ
Resources & Tools
SpectrumViewerDownload MSDSDownload TDS
Preparation and Storage

1. For in vitro diagnostic use.

2. When stored at 2° to 8°C, the antibody reagent is stable until the expiration date shown on the label. Do not use after the expiration date.

3. The antibody reagent should not be frozen or exposed to direct light during storage or during incubation with cells. Keep the reagent vial dry.

4. Alteration in the appearance of the reagent, such as precipitation or discoloration, indicates instability or deterioration. In such cases, the reagent should not be used.

5. The antibody reagent contains sodium azide as a preservative; however, care should be taken to avoid microbial contamination, which may cause erroneous results.

  1. Brenner M, Groh V, Porcelli F, Hochstenbach H, et al. Structure and distribution of the human gd T-cell receptor. In: Knapp W, Dörken B, Gilks W, et al, eds. Leucocyte Typing IV: White Cell Differentiation Antigens. 1989:1049-1053.

  2. Clevers H. Alarcón B, Wileman T, Terhorst C. The T cell receptor/CD3 complex: a dynamic protein ensemble. Annual Rev Immunol. 1988;(6):629.

  3. Edelman G. Cell adhesion molecules. Science. 1983;219:450-457.

  4. Fitzgerald-Bocarlsy P, Herberman R, Hercend T, et al. A definition of natural killer cells. In: Ades E, Lopez C, ed. Natural Killer Cells and Host Defense. 82,8756944444444

  5. Giorgi J. Lymphocyte subset measurements: significance in clinical medicine. In: Rose N, Friedman H, Fahey J, eds. Manual of Clinical Laboratory Immunology. Washington, DC: American Society for Microbiology; 1986:236-246.

  6. Haynes B. β2-interferon/B Leukocyte Typing II: Human T Lymphocytes. 1986:3-30.

  7. Jackson A, Warner N. Preparation, staining, and analysis by flow cytometry of peripheral blood leukocytes. In: Rose N, Friedman H, Fahey J, eds. Manual of Clinical Laboratory Immunology. Washington, DC: American Society for Microbiology; 1986:226-235.

  8. Kan E, Wang C, Wang L, Evans R. Noncovalently bonded subunits of 22 and 28 kd are rapidly internalized by T cells reacted with Anti-Leu-4 antibody. J Immunol. 1983;131:536-539.

  9. Knapp W, Reiber P, Dörken B, Schmidt R, Stein H, von dem Borne AEGKr. Towards a better definition of leucocyte surface molecules. Immunol Today. 1989;10:253-258.

  10. Knowles R. Immunochemical analysis of the T cell-specific antigens. In: Reinherz E, Haynes B, Nadler L, Bernstein I, eds. Leukocyte Typing II: Human T Lymphocytes. 1986:259-288.

  11. Lanier L, Chang C, Azuma M, Ruitenberg J, Hemperly J, Phillips J. Molecular and functional analysis of human natural killer cell-associated neural cell adhesion molecule (N-CAM/CD56). J Immunol. 1991;146:4421-4426.

  12. Lanier L, Le A, Civin C, Loken M, Phillips J. The relationship of CD16 (Leu-11) and Leu-19 (NKH- 1) antigen expression on human peripheral blood NK cells and cytotoxic T lymphocytes. J Immunol. 1986;136:4480-4486.

  13. Lanier L, Testi R, Bindl J, Phillips J. Identity of the Leu-19 (CD56) leucocyte differentiation antigen and neural-cell adhesion molecule. J Exp Med. 1989;169:2233-2238.

  14. Ledbetter J, Evans R, Lipinski M, Cunningham-Rundles C, RA G, Herzenberg L. Evolutionary conservation of surface molecules that distinguish T lymphocyte helper/inducer and T cytotoxic/ suppressor subpopulations in mouse and man. J Exp Med. 1981;153:310-323.

  15. National Committee for Clinical Laboratory Standards. Clinical Applications of Flow Cytometry: Quality Assurance and Immunophenotyping of Peripheral Blood Lymphocytes; Tentative Guideline. (H42-T). 1992

  16. National Committee for Clinical Laboratory Standards. Procedures for the Collection of Diagnostic Blood Specimens by Venipuncture (H3-A3). 1991

  17. Perussia B, Acuto O, Terhorst C, et al. Human natural killer cells analyzed by B73.1, a monoclonal antibody blocking Fc receptor functions. II. Studies of B73.1 antibody-antigen interaction on the lymphocyte membrane. J Immunol. 1983;130:2142-2148.

  18. Perussia B, Starr S, Abraham S, Fanning V, Trinchieri G. Human natural killer cells analyzed by B73.1, a monoclonal antibody blocking Fc receptor functions. I. Characterization of the lymphocyte subset reactive with B73.1. J Immunol. 1983;130:2133-2141.

  19. Perussia B, Trinchieri G, Jackson A, et al. The Fc receptor for IgG on human natural killer cells: Phenotypic, functional, and comparative studies with monoclonal antibodies. J Immunol. 1984;133:180-189.

  20. Phillips J, Lanier L. Dissection of the lymphokineactivated killer phenomenon: Relative contribution of peripheral blood natural killer cells and T lymphocytes to cytolysis. J Exp Med. 1986;164:814-825.

  21. Prince H, Hirji K, Waldbeser L, Plaeger-Marshall S, Kleinman S, Lanier L. Influence of racial background on the distribution of T cell subsets and Leu-11-positive lymphocytes in healthy blood donors. Diag Immunol. 1985;3:33-37.

  22. Reichert T, DeBruyère M, Deneys V, et al. Lymphocyte subset reference ranges in adult Caucasians. Clin Immunol Immunopathol. 1991;60:190-208.

  23. Schmidt R. Non-lineage/natural killer section report: New and previously defined clusters. In: Knapp W, Dörken B, Gilks W, et al, eds. Leucocyte Typing IV: White Cell Differentiation Antigens. 1989;517-542.

  24. Schubert J, Lanier L, Schmidt R. Cluster report: CD56. In: Knapp W, Dörken B, Gilks W, et al, eds. Leucocyte Typing IV: White Cell Differentiation Antigens. 1989;699-702.

  25. van Dongen J, Krissansen G, Wolvers-Tettero I, et al. Cytoplasmic expression of the CD3 antigen as a diagnostic marker for immature T-cell malignancies. Blood. 1988;71:603-612.

BD Biosciences提供了广泛的试剂组合，用于研究细胞凋亡，细胞周期和细胞增殖。已经开发了几种方法来评估各种样品中的细胞凋亡，细胞周期和细胞增殖。我们的试剂可以探索这些过程的细胞特征。此外，BD Biosciences提供了多种工具来协助客户进行实验设置和分析。