BD Biosciences/Anti-Human CD4 FITC/CD38 PE/CD3 PerCP/HLA-DR APC/20 µL/340573

价格
面议
货号:340573
浏览量:127
品牌:BD Biosciences
服务
全国联保
正品保证
正规发票
签订合同
商品描述
Product DetailsRecommended AssayReferences

Description

CD4, clone SK3, is derived from hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human peripheral blood T lymphocytes.

CD38, clone HB7, is derived from hybridization of mouse P3-X63-Ag8.653 myeloma cells with spleen cells from BALB/c mice immunized with the BJAB cell line.

CD3, clone SK7, is derived from hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human thymocytes.

Anti–HLA-DR, clone L243, is derived from the hybridization of mouse NS-1/1-Ag4 myeloma cells with spleen cells from BALB/c mice immunized with the human lymphoblastoid B-cell line RPMI 8866.

CD4 recognizes an antigen that interacts with class II molecules of the major histocompatibility complex (MHC) and is the primary receptor for the human immunodeficiency virus. The cytoplasmic portion of the antigen is associated with the protein tyrosine kinase p56lck. The CD4 antigen can regulate the function of the CD3 antigen/Tcell antigen receptor (TCR) complex. CD4 also reacts with monocytes/macrophages that have an antigen density lower than that on helper/inducer T lymphocytes.

CD38 recognizes an integral membrane glycoprotein, Mr 45 kDalton (kDa), with a protein core of 35 kDa.

CD3 recognizes the epsilon chain of the CD3 antigen/T-cell antigen receptor (TCR) complex. This complex is composed of at least six proteins that range in molecular weight from 20 to 30 kDa. The antigen recognized by the CD3 antibody is noncovalently associated with either α/β or γ/δ TCR (70 to 90 kDa).

Anti–HLA-DR recognizes a human class II MHC antigen. The antigen is a transmembrane glycoprotein composed of α and β subunits that have molecular weights of 36 and 27 kDa, respectively. Anti–HLA-DR reacts with a nonpolymorphic HLA-DR epitope and does not cross-react with HLA-DQ or HLA-DP molecules.

Contents

SpecificityCloneFormatIsotypeEntrez Gene ID
HLA-DRL243APCIgG2a, κ0
CD4SK3 (also known as Leu3a)FITCIgG1, κ920
CD38HB7 (also known as HB-7)PEIgG1, κ952
CD3SK7 (also known as Leu-4)PerCPIgG1, κ916
Preparation and Storage

The MultiTEST reagent is supplied as a combination of CD4 FITC, CD38 PE, CD3 PerCP, and Anti–HLA-DR APC in 1.0 mL of PBS containing bovine serum albumin, and 0.1% sodium azide. Vials should be stored at 2° to 8°C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label when stored as directed.

  1. Benito JM, Zabay JM, Gil J, Bermejo M, Escudero A, Sánchez E, Fernández-Cruz E Quantitative alterations of the functionally distinct subsets of CD4 and CD8 T lymphocytes in asymptomatic HIV infection: Changes in the expression of CD45RO, CD45RA, CD11b, CD38, HLA-DR, and CD35 antigens. J Acquir Immune Defic Syndr Hum Retrovirol. 1997;14:128-135.

  2. Bernard A, Boumsell L, Hill C. Joint report of the first international workshop on human leucocyte differentiation antigens by the investigators of the participating laboratories. In: Bernard A, Boumsell L, Dausset J, Milstein C, Schlossman SF, eds. Leucocyte Typing. New York, NY: Springer-Verlag; 1984:9-108.

  3. Bishop GA, Hall BM, Duggin GG, Horvath JS, Sheil AGR, Tiller DJ. Immunopathology of renal allograft rejection analyzed with monoclonal antibodies to mononuclear cell markers. Kidney Internat. 1986;29:708- 717.

  4. Bogner J, Matuschke B, Hienrich B, Schreiber M, Nerl C, Goebel F. Expansion of activated T lymphocytes (CD3+HLA-DR+) detectable in early stages of HIV-1 infection. Klin Wochenschr. 1990;68:393-396.

  5. Brenner M, Groh V, Porcelli F, Hochstenbach H, et al. Structure and distribution of the human gd T-cell receptor. In: Knapp W, Dörken B, Gilks W, et al, eds. Leucocyte Typing IV: White Cell Differentiation Antigens. 1989:1049-1053.

  6. Brodsky FM. A matrix approach to human class II histocompatibility antigens: reactions of four monoclonal antibodies with the products of nine haplotypes. Immunogenetics. 1984;19:179-194.

  7. Clevers H. Alarcón B, Wileman T, Terhorst C. The T cell receptor/CD3 complex: a dynamic protein ensemble. Annual Rev Immunol. 1988;(6):629.

  8. Dalgleish A, Beverley P, Clapham P, Crawford D, Greaves M, Weiss R. The CD4 (T4) antigen is an essential component of the receptor for the AIDS virus. Nature. December 1984;312:763-767.

  9. Dörken B, Möller P, Pezzutto A, Schwartz-Albiez R, et al. B-cell antigens: CD38. In: Knapp W, Dörken B, Gilks W, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York: Oxford University Press; 1989:86.

  10. Edwards JA, Durant BM, Jones DB, Evans PR, Smith JL. Differential expression of HLA class II antigens in fetal human spleen: relationship of HLADP, DQ, and DR to immunoglobulin expression. J Immunol. 1986;137:490-497.

  11. Engleman EG, Warnke R, Fox RI, Levy R. Studies of a human T lymphocyte antigen recognized by a monoclonal antibody. Proc Natl Acad Sci USA. 1981;78:791.

  12. Evans RL, Wall DW, Platsoucas CD, et al. Thymus-dependent membrane antigens in man: inhibition of cell-mediated lympholysis by monoclonal antibodies to the TH2 antigen. Proc Natl Acad Sci USA. 1981;78:544-548.

  13. Garson JA, Beverly PCL, Coakham HB, Harper EJ. Monoclonal antibodies against human T lymphocytes label Purkinje neurons of many species. Nature. 1982;298:375.

  14. Giorgi J, Hultin L. Lymphocyte subset alterations and immunophenotyping by flow cytometry in HIV disease. Clin Immunol Newslett. 1990;10:55-61.

  15. Haynes B. Summary of T-cell studies performed during the Second International Workshop and Conference on Human Leukocyte Differentiation Antigens. In: Reinherz E, Hayes B, Nadler L, Bernstein I, eds. Leukocyte Typing II: Human T Lymphocytes. 1986:3-30.

  16. Kan E, Wang C, Wang L, Evans R. Noncovalently bonded subunits of 22 and 28 kd are rapidly internalized by T cells reacted with Anti-Leu-4 antibody. J Immunol. 1983;131:536-539.

  17. Knowles R. Immunochemical analysis of the T cell-specific antigens. In: Reinherz E, Haynes B, Nadler L, Bernstein I, eds. Leukocyte Typing II: Human T Lymphocytes. 1986:259-288.

  18. Kurrle R. Cluster report: CD3. In: Knapp W, Dörken B, Gilks WR, et al., eds. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:290-293.

  19. Lampson LA, Levy R. Two populations of Ia-like molecules on a human B cell line. J Immunol. 1980;125:293-299.

  20. Landay A, Ohlsson-Wilhelm B, Giorgi J. Application of flow cytometry to the study of HIV infection. AIDS. 1990;4:479-497.

  21. Lanier LL, Allison JP, Phillips JH. Correlation of cell surface antigen expression on human thymocytes by multi-color flow cytometric analysis: implications for differentiation. J Immunol. 1986;137:2501-2507.

  22. Legendre C, Schiffrin A, Weitzner G, Colle E, Guttman R. Two-color flow cytometry analysis of activated T-lymphocyte subsets in type 1 diabetes mellitus. Diabetes. 1988;37:792-795.

  23. Lenki R, Bratt G, Holmberg V, Muirhead K, Sandstrom E. Indicators of T-cell activation: Correlation between quantitative CD38 expression and soluble CD8 levels in asymptomatic HIV+ individuals and healthy controls. Cytometry. 1998;33:115-122.

  24. Levacher M, Tallet S, Dazza M, Dournon E, Rouveix B, Pocidalo J. T activation marker evaluation in ARC patients treated with AZT: Comparison with CD4+ lymphocyte count in non-progressors and progressors towards AIDS. Clin Exp Immunol. 1990;81:177-182.

  25. Maddon P, Dalgleish A, McDougal J, Clapham P, Weiss R, Axel R. The T4 gene encodes the AIDS virus receptor and is expressed in the immune system and the brain. Cell. 1986;47:333-348.

  26. Nicholson J, Jones B, Hubbard M. CD4 T-lymphocyte determinations on whole blood specimens using a single-tube three-color assay. Cytometry. 1993;14(August):598-605.

  27. Pezzutto A, Behm F, Callard R, et al. Flow cytometry analysis of the B-cell blind panel: Joint Report. In: Knapp W, Dörken B, Gilks W, ed. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:165-174.

  28. Reichert T, De Bruyère M, Deneys V, et al. Lymphocyte subset reference ranges in adult Caucasians. Clin Immunol Immunopath. 1991;60:190-208.

  29. Reinherz E, Kung P, Goldstein G, Levey R, et al. Discrete stages of human intrathymic differentiation: Analysis of normal thymocytes and leukemic lymphoblasts of T-cell lineage. Proc Natl Acad Sci USA. 1980;77:1588-1592.

  30. Robbins PA, Evans EL, Ding AH, Warner NL, Brodsky FM. Monoclonal antibodies that distinguish between class II antigens (HLA-DP, DQ, and DR) in 14 haplotypes. Human Immunol. 1987;18:301-313.

  31. Rudd C, Burgess K, Barber E, Schlossman S. Monoclonal antibodies to the CD4 and CD8 antigens precipitate variable amounts of CD4/CD8-associated p56lck activity. In: Knapp W, Dörken B, Gilks WR, et al, eds. Leucocyte Typing IV: White Cell Differentiation Antigens. New York, NY: Oxford University Press; 1989:326-327.

  32. Salazar-Gonzalez J, Moody D, Giorgi J, Martinez-Maza O, et al. Reduced ecto-5"-nucleotidase activity and enhanced OKT10 and HLA-DR expression on CD8 (T suppressor/cytotoxic) lymphocytes in the acquired immune deficiency syndrome: Evidence of CD8 cell immaturity. J Immunol. 1985;135:1778-1785.

  33. Stites D, Casavant C, McHugh T, et al. Flow cytometric analysis of lymphocyte phenotypes in AIDS using monoclonal antibodies and simultaneous dual immunofluorescence. Clin Immunol Immunopathol. 1986;38:161-177.

  34. Tedder TF, Clement L, Cooper M. Discontinuous expression of a membrane antigen (HB-7) during B lymphocyte differentiation. Tissue Antigens. 1984;24:140-149.

  35. Tedder TF, Crain M, Kubagawa H, Clement L, et al. Evaluation of lymphocyte differentiation in primary and secondary immunodeficiency diseases. J Immunol. 1985;135:1786-1791.

  36. Terstappen L, Hollander Z, Meiners H, Loken M. Quantitative comparison of myeloid antigens on five lineages of mature peripheral blood cells. J Leuk Biol. 1990;48:138-148.

  37. Terstappen L, Huang S, Picker L. Flow cytometric assessment of human T cell differentiation in thymus and bone marrow. Blood. 1992;79:666-677.

  38. Terstappen LWMM, Hollander Z, Meiners H, Loken MR. Quantitative comparison of myeloid antigens on five lineages of mature peripheral blood cells. J Leuk Biol. 1990;48:138-148.

  39. Terstappen LWMM, Huang S, Safford M, Lansdorp PM, Loken MR. Sequential generations of hematopoietic colonies derived from single nonlineage-committed CD34+CD38- progenitor cells. Blood. 1991;77:1218-1227.

  40. Tomkinson BE, Wagner DK, Nelson DL, Sullivan JL. Activated lymphocytes during acute Epstein-Barr virus infection. J Immunol. 1987;139:3802-3807.

  41. van Dongen J, Krissansen G, Wolvers-Tettero I, et al. Cytoplasmic expression of the CD3 antigen as a diagnostic marker for immature T-cell malignancies. Blood. 1988;71:603-612.

  42. Vanham G, Kestens L, Gigase P, et al. Evidence for circulating activated cytotoxic T cells in HIVinfected subjects before the onset of opportunistic infections. Clin Exp Immunol. 1990;82:3-9.

  43. Warnke R, Levy R. Detection of T and B cell antigens hybridoma monoclonal antibodies: a biotin-avidin-horseradish peroxidase method. J Histochem Cytochem. 1980;28:771-776.

  44. Warnke R, Miller R, Grogan T, Pederson M, Dilley J, Levy R. Immunologic phenotype in 30 patients with diffuse large-cell lymphoma. N Eng J Med. 1980;303:293-300.

  45. Wood GS, Warner N, Warnke R. Anti-Leu-3/T4 antibodies react with cells of monocyte/macrophage and Langerhans lineage. J Immunol. 1983;131(1):212-216.

  46. Zipf TF, Fox RI, Dilley J, Levy R. Definition of the high-risk acute lymphoblastic leukemia patient by immunological phenotyping with monoclonal antibodies. Cancer Res. 1981;41:4786-4789.

BD Biosciences提供了广泛的试剂组合,用于研究细胞凋亡,细胞周期和细胞增殖。已经开发了几种方法来评估各种样品中的细胞凋亡,细胞周期和细胞增殖。我们的试剂可以探索这些过程的细胞特征。此外,BD Biosciences提供了多种工具来协助客户进行实验设置和分析。