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ApexBio/Hexa His tag peptide/25mg/A6006

价格
¥10360.00
货号:A6006
浏览量:127
品牌:ApexBio
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商品描述
Related Products
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Molarity CalculatorDilution Calculator
Hexa His tag peptideSynthetic 6XHis peptide

Catalog No.A6006
SizePriceStockQty
5mg
$129.00
In stock
25mg
$518.00
In stock

Tel: +1-832-696-8203

Email: sales@apexbt.com

Worldwide Distributors

Sample solution is provided at 25 µL, 10mM.

Product Citations

1. Chang M, Qin Q, et al. "Carboxymethylated polyethylenimine modified magnetic nanoparticles specifically for purification of His-tagged protein." J Sep Sci. 2018 Nov 28.PMID:304885562. Guo M, Tang X, et al. "Comparative study of the adjuvant potential of four Th0 cytokines of flounder (Paralichthys olivaceus) on an E. tarda subunit vaccine." Dev Comp Immunol. 2018 Sep;86:147-155.PMID:297469823. Bartoschik T, Galinec S, et al. "Near-native,site-specific and purification-free protein labeling for quantitative protein interaction analysis by MicroScale Thermophoresis." Sci Rep. 2018 Mar 21;8(1):4977.PMID:29563556 4. Guo M, Tang X, et al. "The Immune Adjuvant Effects of Flounder (Paralichthys olivaceus) Interleukin-6 on E.tarda Subunit Vaccine OmpV." Int J Mol Sci. 2017 Jul 5;18(7). pii: E1445.PMID:28678171

Quality Control

Quality Control & MSDS

View current batch:
    Purity = 99.90%
  • COA (Certificate Of Analysis)
  • HPLC
  • MS (Mass Spectrometry)
  • MSDS (Material Safety Data Sheet)
  • Datasheet

Chemical structure

Hexa His tag peptide

Related Biological Data

Hexa His tag peptide

Related Biological Data

Hexa His tag peptide

Related Biological Data

Hexa His tag peptide

Biological Activity

DescriptionHexa His tag peptide is a 6 x His tag used as a metal binding site for the recombinant protein.
Targetsanti-6 x His tag antibody
IC50

Hexa His tag peptide Dilution Calculator

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Hexa His tag peptide Molarity Calculator

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Chemical Properties

Cas No. N/ASDF Download SDF
Synonyms H-His-His-His-His-His-His-OH
Chemical Name Hexa His
Canonical SMILES C1=C(NN=C1)CC(C(=O)NC(CC2=CC=NN2)C(=O)NC(CC3=CC=NN3)C(=O)NC(CC4=CC=NN4)C(=O)NC(CC5=CC=NN5)C(=O)NC(CC6=CC=NN6)C(=O)O)N
Formula C36H44N18O7 M.Wt 840.85
Solubility ≥84.1mg/mL in DMSO Storage Desiccate at -20°C
Shipping ConditionEvaluation sample solution : ship with blue ice.All other available size:ship with RT , or blue ice upon request
General tipsFor obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.

Background

Hexa His is an amino acid sequence consisting of 6 His residues in a row, it is also known as hexa histidine-tag, 6xHis-tag, and by the trademarked name His-tag. Hexa His Responsible for the degradation of GM2 gangliosides, and a variety of other molecules containing terminal N-acetyl hexosamines, in the brain and other tissues. The form B is active against certain oligosaccharides. The form S has no measurable activity. Hexa His acts as a metal binding site for a recombinant protein. His-Tag sequence can be placed on the N- or -terminal of a target protein by using vectors from various commercial molecular biology companies. And this Tag is often followed by a cleavage site for a specific protease - for example Leu-Val-Arg-Gly-Ser peptide sequence is recognized and cleaved by the protease known as thrombin.

References: 1.  Hengen, P (1995). "Purification of His-Tag fusion proteins from Escherichia coli". Trends in Biochemical Sciences 20 (7): 285–6.

ApexBio的3X FLAG Peptide FLAG标签系统利用与目标蛋白质1融合的短而亲水的8个氨基酸的肽段。FLAG肽与抗体M1结合。结合是钙依赖性方式2还是非依赖性3仍存在争议。该系统的缺点是单克隆抗体纯化基质不如其他基质稳定。通常,可以用特异性单克隆抗体检测小标签。为了改善对FLAG标签的检测,已经开发了3x FLAG系统。这种三级FLAG表位是亲水的,长22个氨基酸,可以检测到高达10 fmol的表达融合蛋白。激烈热球菌的带有FLAG标签的麦芽糖糊精结合蛋白已被结晶4,其晶体质量与未标记蛋白的晶体质量非常相似。 最后,可以通过用肠激酶处理去除FLAG标签,肠激酶对肽序列5的5个C末端氨基酸具有特异性。