| CID 2745687GPR35 antagonist |
Sample solution is provided at 25 µL, 10mM.
Nature.2017 Jan 19;541(7637):417-420.
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| Cas No. | 264233-05-8 | SDF | Download SDF |
| Synonyms | N/A | ||
| Chemical Name | (E)-methyl 5-((2-(tert-butylcarbamothioyl)hydrazono)methyl)-1-(2,4-difluorophenyl)-1H-pyrazole-4-carboxylate | ||
| Canonical SMILES | S=C(N/N=C/C1=C(C(OC)=O)C=NN1C(C(F)=C2)=CC=C2F)NC(C)(C)C | ||
| Formula | C17H19F2N5O2S | M.Wt | 395.43 |
| Solubility | Soluble in DMSO | Storage | Store at -20°C |
| Physical Appearance | A crystalline solid | Shipping Condition | Evaluation sample solution : ship with blue ice.All other available size:ship with RT , or blue ice upon request |
| General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months. | ||
pIC50: 6.70 for human GPR35
CID-2745687 is a GPR35 antagonist. GPR35 is a poorly characterized member of the rhodopsinlike, class A subfamily of G protein-coupled receptors (GPCRs). GPCRs, based on the expression pattern, has been considered as a possible therapeutic target in conditions including diabetes, cardiovascular disease, as well as inflammation and pain.
In vitro: Previous study indicated that both CID-2745687 and ML-145 could competitively inhibit the effects of cromolyn disodium and zaprinast (two agonists sharing an overlapping binding site) on human GPR35. In contrast, though ML-145 antagonized the effects of pamoate competitively, CID-2745687 showed a noncompetitive fashion. Additionally, neither ML-145 nor CID-2745687 was able to antagonize the agonist effects at rodent ortholog of GPR35 [1].
In vivo: To test whether GPR35 contributes to the metabolic effect of Zaprinast, the retina from Cngb1/ mice was preincubated with a GPR35 antagonist, CID-2745687, followed by an additional Zaprinast treatment. Results showed that CID-2745687 did not block the effect of Zaprinast on glutamate and aspartate. Moreover, pamoic acid, the GPR35 agonist, did not change aspartate or glutamate levels [1].
Clinical trial: N/A
References:[1] Jenkins L,Harries N,Lappin JE,MacKenzie AE,Neetoo-Isseljee Z,Southern C,McIver EG,Nicklin SA,Taylor DL,Milligan G. Antagonists of GPR35 display high species ortholog selectivity and varying modes of action. J Pharmacol Exp Ther.2012 Dec;343(3):683-95. [2] Du J,Cleghorn WM,Contreras L,Lindsay K,Rountree AM,Chertov AO,Turner SJ,Sahaboglu A,Linton J,Sadilek M,Satrústegui J,Sweet IR,Paquet-Durand F,Hurley JB. Inhibition of mitochondrial pyruvate transport by zaprinast causes massive accumulation of aspartate at the expense of glutamate in the retina. J Biol Chem.2013 Dec 13;288(50):36129-40.
