Cell lines

MDCK cells

Preparation method

The solubility of this compound in DMSO is limited. General tips for obtaining a higher concentration: Please warm the tube at 37 °C for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below - 20 °C for several months.

Reacting condition

0 ~ 12 μM

Applications

Oseltamivir Phosphate exhibited inhibitory effects against both H5N1 and H9N2 viruses. In MDCK cells, the active metabolite of Oseltamivir Phosphate reduced viral replication with the EC50 values ranging from 7.5 to 12 μM, and reduced neuraminidase activity with the IC50 values ranging from 7.0 to 15 nM.

Animal models

Mice infected with A/HK/156/97 (H5N1) or A/Qa/HK/G1/97 (P3)

Dosage form

0.1, 1, 10 and 100 mg/kg/d; p.o.

Applications

In mice infected with H5N1, Oseltamivir Phosphate significantly decreased the titer of virus in the lungs. However, the reduction in virus titer was almost the same as between the 1 and 10 mg/kg/d dose groups and the 100 mg/kg/d dose group. In mice infected with P3, Oseltamivir Phosphate at the doses of 1 and 10 mg/kg/d also significantly reduced the virus titer in lungs, and a dose of 100 mg/kg/d Oseltamivir Phosphate completely inhibited virus replication.

Other notes

Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.

References:

[1]. Leneva IA, Roberts N, Govorkova EA, Goloubeva OG, Webster RG. The neuraminidase inhibitor GS4104 (oseltamivir phosphate) is efficacious against A/Hong Kong/156/97 (H5N1) and A/Hong Kong/1074/99 (H9N2) influenza viruses. Antiviral Res. 2000 Nov;48(2):101-15.