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蚂蚁淘在线 / 品牌中心 / pblassaysci / PBL/Human IFN Gamma ELISA Kit (TCM)/1 x 96-well plate/41500-1

PBL/Human IFN Gamma ELISA Kit (TCM)/1 x 96-well plate/41500-1

价格
￥7000.00
货号：41500-1
浏览量：127
品牌：pblassaysci
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商品描述

Human IFN Gamma ELISA Kit (TCM)

Home / Products / Assay Kits / ELISAs / Human Interferon ELISAs / Human IFN Gamma ELISA Kit (TCM)
Product Features:
  • Quantifies IFN Gamma in tissue culture media (TCM)

Full Product Name: VeriKine Human Interferon Gamma ELISA Kit

 

Ordering

Catalog No. Pack Size Price Quantity
41500-1

1 x 96-well plate

$350.00
  • Specifications
  • Tech Info / Data
  • Citations
  • Documentation

Specifications

Compatibility: Tissue Culture Media
Assay Range: 12.5 - 500 pg/ml
Speed: Incubation time, 3 hours, 15 minutes
Specificity: Human Interferon Gamma (IFN-gamma, IFNG)
No cross-reactivity against human IFN-α or IFN-β; mouse or rat IFN-α, IFN-β or IFN-γ.
 
Storage: 2-8°C
Expiration Date: One year from date of manufacture
Shipping Condition: Wet Ice

Materials Provided:

  • Pre-coated microtiter plate
  • Plate Sealers
  • Wash Solution Concentrate
  • Human Interferon Gamma Standard, 10,000 pg/ml
  • Dilution Buffer 
  • Antibody Concentrate 
  • HRP Conjugate Concentrate 
  • Assay Diluent
  • TMB Substrate Solution
  • Stop Solution 

Tech Info / Data

Application Note:

  • Interferon Gamma Activity and ELISA Detection Comparison
Background:

Human IFN Gamma ELISA kit for quantitative measurement of human interferon gamma (IFNG-γ) in tissue culture media. This kit is based on an ELISA with anti-detection antibody conjugated to horseradish peroxidase (HRP). Tetramethyl-benzidine (TMB) is the substrate. All reagents are supplied. One pre-coated microtiter plate (96-well) is included. The assay is based on the international reference standard for human interferon gamma (Hu-IFN-γ) provided by the National Institutes of Health. Typical standard curves for each lot are included with the procedure.

Interferon Gamma (IFN-gamma or IFNG) is a pleiotropic cytokine secreted by Natural Killer (NK) cells and many T cells. It is the sole representative of Type II IFN. IFN-gamma has pleiotropic activities including: inducing an antiviral state in cells, upregulating cell surface markers such as Class I and II MHC, and regulating the activity of macrophages, NK cells, neutrophilis, B cells and T cells.

Citations

3 Citations:

  1. Colavita, Francesca, et al. (2018). Overproduction of IL-6 and Type-I IFN in a Lethal Case of Chikungunya Virus Infection in an Elderly Man During the 2017 Italian Outbreak. Open Forum Infectious Diseases, 21 pgs. PMID: 30539034. (link)

  2. Diaz-Rodriguez, Yildian, et al. (2017). In Vitro Differentiated Plasmacytoid Dendritic Cells as a Tool to Induce Anti-Leukemia Activity of Natural Killer Cells. Cancer Immunology, Immunotherapy, 14 pgs. PMID: 28555259. (link)

  3. Oon, Shereen, et al. (2016). A Cytotoxic Anti-IL-3R-alpha Antibody Targets Key Cells and Cytokines Implicated in Systemic Lupus Erythematosus. JCI Insight, 15 pgs. PMID: 27699260. (link)

References:

  1. Staehelin et al. (1981). Methods in Enzymology, Vol. 79 (Pestka, ed.), Academic Press, New York, 589-595.

  2. Kelder et al. (1986). Methods in Enzymology, Vol. 119 (Pestka, ed.), Academic Press, New York, 582-587.

  3. Human IFN-α international reference standard provided by the NIH, reference no. Gg23-901-530. Pestka (1986). Methods in Enzymology, Vol. 119 (Pestka, ed.), Academic Press, New York, 14-23.

  4. Kung et al. (1986). Methods in Enzymology, Vol. 119. (Pestka, ed.), Academic Press, New York, 204-210.

Documentation

PDF icon 41500 Protocol
PDF icon 41500 Material Safety Data Sheet (MSDS)

Typical IFN-gamma Standard Curve from 12.5 to 500 pg/ml using PBL"s Human IFN-γ ELISA for TCM (41500)

Typical IFN-γ Standard Curve from 12.5 to 500 pg/ml using PBL's Human IFN-γ ELISA for TCM (41500)

Assay Range: 12.5 - 500 pg/ml

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