The VeriKine Human IFN Gamma Receptor 1 ELISA quantitates Human IFN Gamma Receptor 1 (IFNGR1)  in serum, plasma, tissue culture media (TCM) and cell lysates by sandwich enzyme linked immunosorbent assay (ELISA). IFNGR1 binds to plates coated with antibody and detection is accomplished using a detection antibody followed by streptavidin conjugated to horseradish peroxidase (HRP). This ELISA kit utilizes Tetramethyl-benzidine (TMB) as the substrate. The standard provided is recombinant Human IFNGR1 expressed in mammalian cells.

The receptor for interferon gamma (IFN-gamma) is a membrane bound heterodimer of the IFNGR1 and IFNGR2 chain with IFNGR1 providing the high affinity binding site. IFNGR1 is also known as CD119 and Interferon Gamma Receptor alpha (IFNGRa). Binding of IFN-gamma to the receptor leads to activation of a number of genes and multiple biological activities. The receptor density can vary from 1,000 - 40,000 copies per cell depending upon the cell line. 

IFNGR1 can be present in two forms, the classical membrane bound form and a soluble form. Unlike IFNAR2 no alternatively spliced secreted transcript has been identified for IFNGR1 but is thought to be cleaved at the membrane by proteases in a manner similar to other cytokine receptors. There appears to be a basal level in the serum of healthy donors. The soluble IFNGR1 may be elevated in certain disease states such as Rheumatoid Arthritis and Graft vs. Host Disease.