Product Features:

Contains 12 distinct human IFN alpha subtypes for differential activity studies
cDNAs of interferons were obtained from human leukocyte mRNA, and expressed in E.coli
Provides 100,000 units of IFNa-A, IFNa-B2, IFNa-C, IFNa-D (1[Val¹¹⁴]), IFNa-F, IFNa-G, IFNa-H2, IFNa-I, IFNa-J1, IFNa-K, IFNa-4b, and IFNa-WA (corresponding to IFNa-2a, IFNa-8, IFNa-10, IFNa-1, IFNa-21, IFNa-5, IFNa-14, IFNa-17, IFNa-7, IFNa-6, IFNa-4b, IFNa-16, respectively)

Catalog No.	Pack Size	Price	Quantity
11002-1	1 x 10⁵ units	$2,195.00

Specifications
Tech Info / Data
Citations
Documentation

Specifications

Formulation	Individual interferons are supplied frozen in phosphate-buffered saline (PBS) containing 0.1% Bovine Serum Albumin (BSA)
Molecular Weight	19.2 -19.7 kDa
Source	cDNA of interferons were obtained from human leukocyte mRNA, and expressed in E.coli
Purity	> 95% by SDS-PAGE stained by Coomassie Blue
Bioactivity	Activities of the interferons are measured using a cytopathic inhibition assay on Bovine Kidney Cells (MDBK) with Vesicular Stomatis Virus (VSV); human lung carcinoma cell line A549 with encephalomyocarditis virus (EMCV)
Storage	For retention of full activity store at -70 ºC or below and avoid repeated freeze-thaw cycles
Synonyms	Interferon alpha subtypes, IFN alpha subtypes, IFN-a subtypes, interferon alpha, Leukocyte interferon subtypes, Leukocyte IFN subtypes, alpha interferon subtypes, interferon alfa subtypes, IFN alfa, interferon a, Type I interferon alpha, Type I IFN alpha, Human interferon alpha, IFN-alpha, INF alpha, Human IFN alpha 2a, human IFN alpha 8, human IFN alpha 10, human IFN alpha 1, human IFN alpha 21, human IFN alpha 5, human IFN alpha 14, human IFN alpha 17, human IFN alpha 7, human IFN alpha 6, human IFN alpha 4b, human IFN alpha 16
Accession Number	Refer to CoA

Tech Info / Data

Background:

The Human IFN Alpha Sampler Set provides a collection of 12 distinct biologically active alpha interferons which enables studies of their activity in various applications.

The Human interferon alpha protein family consists of 12 individual protein species, each with reported different biological properties. Some have proven to be 10 and 20 times more effective than Human IFN-alpha A in protecting a cell against specific viruses. Elucidating the functional significances of various IFN-alpha subtypes not only can lead to a better understanding on the role interferons have in an immunological event, but can also lead to the refinement of current interferon based therapeutic applications.

Human IFN Alpha Subtype Nomenclature		Gene
IFN Alpha A	IFN Alpha 2a	IFNA2
IFN Alpha 2	IFN Alpha 2b	IFNA2
IFN Alpha B2	IFN Alpha 8	IFNA8
IFN Alpha C	IFN Alpha 10	IFNA10
IFN Alpha D [Val114]	IFN Alpha 1	IFNA1
IFN Alpha F	IFN Alpha 21	IFNA21
IFN Alpha G	IFN Alpha 5	IFNA5
IFN Alpha H2	IFN Alpha 14	IFNA14
IFN Alpha I	IFN Alpha 17	IFNA17
IFN Alpha J1	IFN Alpha 7	IFNA7
IFN Alpha K	IFN Alpha 6	IFNA6
IFN Alpha 1 [Ala114]	IFN Alpha D	IFNA1
IFN Alpha 4a	IFN Alpha M1	IFNA4
IFN Alpha 4b	IFN Alpha 4	IFNA4
IFN Alpha WA	IFN Alpha 16	IFNA16

For more information on Human IFN Alpha subtype molecular weight, and theoretical pI click here.

Citations

9 Citations:

Prevost, Jeremie, et al. (2019). Upregulation of BST-2 by Type I Interferons Reduces the Capacity of Vpu To Protect HIV-1-Infected Cells from NK Cell Responses. mBio, 18 pgs. PMID: 31213558. (link)

Schlaepfer, et al. (2019). Dose-Dependent Differences in HIV Inhibition by Different Interferon Alpha Subtypes While Having Overall Similar Biologic Effects. mSphere, 14 pgs. PMID: 30760614. (link)

Cook, Bradley, et al. (2016). Limited Effects of Type I Interferons on Kyasanur Forest Disease Virus in Cell Culture. PLOS Neglected Tropical Diseases, 19 pgs. PMID: 27479197. (link)

Lavender, Kerry, et al. (2016). Interferon Alpha Subtype-Specific Suppression of HIV-1 Infection in Vivo. JVI, 13 pgs. PMID: 27099312. (link)

Todt, Daniel, et al. (2016). Antiviral Activities of Different Interferon Types and Subtypes Against Hepatitis E Virus Replication. Antimicrobial Agents and Chemotherapy, 8 pgs. PMID: 26787701. (link)

Harper, Michael, et al. (2015). Interferon-alpha Subtypes in an Ex Vivo Model of Acute HIV-1 Infection: Expression, Potency, and Effector Mechanisms. PLOS Pathogens, 24 pgs. PMID: 26529416. (link)

Schanen, C, et al. (2006). Correlation Between the Anti-Viral-Induced Cytopathic Effect of Interferon-alpha Subtypes and Induction of MxA Protein In Vitro. Microbiology and Immunology, 19 pgs. PMID: 16428869. (link)

Francois, Catherine, et al. (2005). Quantification of Different Human Alpha Interferon Subtypes and Pegylated Interferon Activities by Measuring MxA Promoter Activation. Antimicrobial Agents and Chemotherapy, 6 pgs. PMID: 16127052. (link)

Sperber, Steven, et al. (1992). Anti-HIV-1 activity of recombinant and hybrid species of interferon-alpha. Journal of Interferon Research, 6 pgs. PMID: 1331260. (link)

References:

For more information on the various alleles of IFN alpha 2a, 2b, 4a, 4b, 1 and D.

Gewart, DR., et al. (1995). J Interferon Cytokine Res. 15 (5):403

Hussain, M., et al. (1997). J Interferon Cytokine Res. 17 (9):559

Hussain, M., et al. (2000). J Interferon Cytokine Res. 20:763

Documentation

PBL/Human IFN Alpha Sampler Set/1 x 105 units/11002-1

Human IFN Alpha Sampler Set

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Specifications

Tech Info / Data

Citations

Documentation

PBL/Human IFN Alpha Sampler Set/1 x 10⁵ units/11002-1