Test Label: FAM-labeled target probeTest Format: AmplifyRP® XRT
AmplifyRP XRT for HLVd is a rapid RNA amplification and detection platform designed for field-based or laboratory testing of hops and Cannabis spp (including industrial hemp) for Hop latent viroid. This kit includes lyophilized reaction pellets containing the necessary reagents to amplify HLVd RNA at a single operating temperature (42 °C).
AmplifyRP XRT is a real-time isothermal nucleic acid amplification and detection system that rapidly amplifies small portions of DNA or RNA and offers unrivaled detection capabilities in an easy-to-use testing format. It offers comparable sensitivity and specificity to published PCR methods while eliminating laborious and costly nucleic acid extractions.
The test can be performed virtually anywhere using the battery operated AmpliFire fluorometer. Assay parameters are loaded via barcode and results are automatically displayed as ( + ) or ( - ).
Prior molecular diagnostic experience is not required to perform AmplifyRP XRT tests.
NOTE: This assay requires a fluorometer to work properly.
Limit of Detection (LOD): Approximately 20 fg RNA transcripts
Specificity:
- Inclusive: Designed to detect all HLVd isolates in hops.
- Exclusive: Does not detect: Apple mosaic virus (ApMV), Arabis mosaic virus (ArMV), Citrus bark cracking viroid (CBCVd), Coconut cadang-cadang viroid (CCCVd), Coconut tinangaja viroid (CTiVd), Columnea latent viroid (CLVd), Hop latent virus (HpLV), Hop mosaic virus (HpMV), Hop stunt viroid (HSVd), Potato spindle tber viroid (PSTVd), Tomato chlorotic dward viroid (TCDVd)
Host Limitations: None
Total Assay Time: Less than 30 minutes when used with the AmpliFire as a real-time assay.
Includes:
- XRT reaction pellets for HLVd (48)
- Pre-filled 100 µL PD1 Pellet diluent tubes (48)
- GEB-filled mesh extraction bags (50)
- User Guide
NOTE: This assay requires a fluorometer to work properly.
