Description

Fibrin Fragment E Polyclonal Antibody – HRP Conjugated

Affinity’s Fibrin Fragment E Polyclonal Antibody – HRP Conjugated is the base level of our horseradish peroxidase conjugated Fibrin Fragment E antibodies.  The purity of IgG is typically 90% and is provided in a solution of HEPES buffered saline containing 50% glycerol (v/v).  The titre is essentially the same as the starting antiserum and each vial typically contains the amount of IgG recovered from one milliliter of antiserum however this IgG has been conjugated with Horseradish Peroxidase as an enzyme reporter.  This Fibrin Fragment E Polyclonal Antibody – HRP Conjugated is generally intended for use as labeled primary antibodies in applications such as immunoassay and immunoblotting.

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Product Code: SAFNE-HRP

Retail Product Size:  0.2mg vial

Host Animal: Sheep Anti-Human Fibrin Fragment E Polyclonal Antibody – HRP Conjugated

Species Cross Reactivity: View Chart

Product Datasheet: Fibrin Fragment E Polyclonal Antibody, hrp conjugated anti-human Sheep IgG

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Description of Fibrinogen

Most of the fibrinogen in the circulation consists of 2 copies of each chain (Aα2, Bβ2, γA2), but in normal plasma approximately 10% of the fibrinogen molecules contain one γA chain and one variant γ chain (termed γ′), in which the c-terminal AGDV residues are replaced with the amino acid sequence VRPEHPAETEYDSLYPEDDL.  This variant fibrinogen is commonly referred to as fibrinogen gamma prime (γA/γ′) but has also been called fibrinogen 2 or peak 2 fibrinogen because it elutes separately from fibrinogen 1 (γA2) by ion exchange chromatography.  Residues 414-427 of the γ′ chain of fibrin gamma prime (contain a high-affinity binding site for exosite II of thrombin, which allows the active site of bound thrombin to remain available to interact with substrates while demonstrating resistance to heparin mediated inhibition by antithrombin1-4.

References and Reviews

1. Hantgan RR, Francis CW, Marder VJ; Fibrinogen Structure and Physiology; in Hemostasis and Thrombosis, 3rd Edition, eds. RW Colman, J Hirsh, VJ Marder and EW Salzman, pp 277-300, J.B. Lippincott Co., Philadelphia PA, USA, 1994.

2. Binnie CG, Lord ST; The Fibrinogen Sequences that Interact with Thrombin;  Blood 81,  pp 3186-3192, 1993.

3. Pospisil CH, Stafford AR, Fredenburgh JC, Weitz JI; Evidence that both Exosites on Thrombin Participate in Its High Affinity Interaction with Fibrin; JBC 278, pp 21584-21591, 2003.

4. Medved L, Weisel JW; Recommendations for Nomenclature on Fibrinogen and Fibrin; JTH 7, pp 355-359, 2009.

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