IBPF12, IBPF16, IBPF20, IBPF87Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with human IGFBP-5 recombinant fragment expressed in E. coli corresponding to amino acid residues Lys164-Glu272 (MAb IBPF87), synthetic peptide from human IGFBP-5 corresponding to amino acid residues Cys100-Glu120 (MAbs IBPF12 and IBPF16) or synthetic peptide from human IGFBP-5 corresponding to amino acid residues Ser125-Ile144 (MAb IBPF20).

Human IGFBP-5

IgG1 for MAbs IBPF12, IBPF16, IBPF20IgG2a for MAb IBPF87

All MAbs work in direct ELISA and Western Blotting.Recommended pairs for IGFBP-5 immunodetection in sandwich immunoassay (capture-detection):IBPF12 – IBPF87IBPF16 – IBPF20 MAbIBPF16 works in IGFBP-5 immunoprecepitation.MAbs IBPF16 and IBPF87 can be used for immunoaffinity purification.

Chromatography on protein A Sepharose

PBS, pH 7.4, 0.1 % sodium azide (NaN3)

+ 4 °C

Some applications in which these products may be used are covered by patents issued and applicable in certain countries. Because purchase of these products does not include a license to perform any patented application, users of these products may be required to obtain a patent license, depending on the particular application and country in which the product is used.

This product is sold as an antibody preparation for research use only. Standard Laboratory Practices should be followed when handling this material.Contains sodium azide (0.1 %) as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.