General Category:

Monoclonal antibodies » Neuroscience antibodies

Disease Category:

Neuroscience » Monoclonal Antibodies

MAbs:

GFAP15cc, GFAP81cc, GFAP83cc, GFAP94cc, GFAP98cc, GF5Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with native human GFAP (MAbs GFAP81cc, GFAP83cc, GFAP94cc and GFAP98cc, GF5) or with synthetic peptide ₁AGFKETRASERAEMME₁₆ corresponding to 60-75 a.a.r. of GFAP conjugated with a carrier protein (MAb GFAP15cc).

Specificity:

Human GFAP

MAb Isotypes:

IgG1 for MAbs GFAP15cc, GFAP81cc, GFAP83cc, GFAP94cc, GFAP98ccIgG2b for MAb GF5

Applications:

Recommended pairs for sandwich immunoassay (capture – detection): GFAP83cc – GFAP81cc, GFAP94cc – GFAP98cc, GFAP15cc – GFAP81ccMAbs GFAP15cc, GFAP81cc, GFAP83cc, GFAP94cc and GFAP98cc are recommended for Western blotting.MAbs GFAP15cc, GFAP81cc, GFAP83cc and GF5 are recommended for immunohistochemistry.MAb GF5 is working in ELISA and indirect immunofluorescence staining.

Purification:

Chromatography on protein A Sepharose (MAbs GFAP15cc, GFAP81cc, GFAP83cc, GFAP94cc, GFAP98cc)Chromatography on protein G Sepharose (MAb GF5)

Presentation:

PBS, pH 7.4, 0.09 % sodium azide (NaN₃)

Storage Conditions:

+4 °C (+2 … +8 °C allowed)

Material Safety Note:

This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.

Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

Advanced immunochemical高敏C反应蛋白人C反应蛋白（CRP）是所谓的急性期蛋白之一。它在血液中的浓度随着对炎症的反应而迅速增加。CRP是224个残基蛋白，单体分子量约为25 kDa，pI 6.4（1-4）。它属于五味子毒素，这是一种进化保守的蛋白质家族，其特征在于钙依赖性配体结合和五个单体的对称性，这些单体在中心孔周围形成一个环（5）。CRP在体内的确切功能尚不完全清楚。已证明CRP参与炎症以及先天免疫过程。CRP的重要生物活性取决于其与各种配体（例如受损细胞膜，凋亡细胞和纤连蛋白）的结合能力，对磷胆碱残基的亲和力最高。当CRP与配体结合时，它可以被补体成分C1q识别，从而激活经典补体途径。另一方面，CRP通过与补体因子H相互作用来调节替代补体途径