MAbs:
PL4cc, PL26cc, PL42cc, PL46cc
Hybridoma clones have been derived from hybridization of Sp2/0 myeloma cells with spleen cells of Balb/c mice immunized with recombinant human Lp-PLA2.
MAb Isotypes:
IgG1 for MAbs PL4cc, PL26cc, PL42cc, PL46cc
Applications:
Sandwich immunoassay, Western blottingRecommended pairs for Lp-PLA2 sandwich immunoassay for detecting Lp-PLA2 in human serum and plasma (capture – detection):PL42cc – PL46ccPL26cc – PL46ccPL26cc – PL4ccMAb PL26cc reacts with Lp-PLA2 in Western blotting.
Purification:
Chromatography on protein A Sepharose
Presentation:
PBS, pH 7.4, 0.09 % sodium azide (NaN₃)
Storage Conditions:
+4 °C (+2 … +8 °C allowed)
Recommendation:
Replace bovine serum albumin (BSA) with 1% casein as a buffer component or to use blocking agent in Lp-PLA2 immunoassay.Blocking of the immunoassay plates with casein is needed to prevent non-specific binding of Lp-PLA2 to the plate surface.
Material safety note:
This product is sold for research use only. Standard Laboratory Practices should be followed when handling this material.Product contains sodium azide as a preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.