- SynonymRING finger protein 43,RING-type E3 ubiquitin transferase RNF43
- SourceHuman RNF43, Fc Tag (RN3-H5256) is expressed from human 293 cells (HEK293). It contains AA Gln 44 - Tyr 197 (Accession # Q68DV7-1).Predicted N-terminus: Gln 44Request for sequence
- Molecular Characterization
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 43.5 kDa. The protein migrates as 50-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>95% as determined by SDS-PAGE.
- Formulation
Please contact us for detailed information.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human RNF43, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human RNF43, Fc Tag (Cat. No. RN3-H5256) was more than 85% and the molecular weight of this protein is around 100-115 kDa verified by SEC-MALS.
Immobilized Human R-Spondin 1, His Tag (Cat. No. RS1-H4221) at 5 μg/mL (100 μL/well) can bind Human RNF43, Fc Tag (Cat. No. RN3-H5256) with a linear range of 2-20 ng/mL (QC tested).
Immobilized Human R-Spondin 3 (22-146), His Tag (Cat. No. RS6-H5222) at 5 μg/mL (100 μL/well) can bind Human RNF43, Fc Tag (Cat. No. RN3-H5256) with a linear range of 0.3-2 ng/mL (Routinely tested).
Immobilized Human R-Spondin 1 (21-146), His Tag (Cat. No. RS6-H4220) at 5 μg/mL (100 μL/well) can bind Human RNF43, Fc Tag (Cat. No. RN3-H5256) with a linear range of 0.01-0.156 μg/mL (Routinely tested).
- BackgroundE3 ubiquitin-protein ligase that acts as a negative regulator of the Wnt signaling pathway by mediating the ubiquitination, endocytosis and subsequent degradation of Wnt receptor complex components Frizzled. Acts on both canonical and non-canonical Wnt signaling pathway. Along with RSPO2 and ZNRF3, constitutes a master switch that governs limb specification (By similarity)
- References
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