ACROBiosystems/Human TNFR2 / CD120b / TNFRSF1B Protein, His Tag/50ug/TN2

价格
¥36000.00
货号:TN2
浏览量:38
品牌:ACROBiosystems
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商品描述
  • Synonym
    TNFRSF1B,CD120b,TBPII,TNF-R-II,TNF-R75,TNFBR,TNFR1B,TNFR2,TNFR80,p75,p75TNFR
  • Source
    Human TNFR2, His Tag (TN2-H5227) is expressed from human 293 cells (HEK293). It contains AA Leu 23 - Asp 257 (Accession # AAA36755).
    Predicted N-terminus: Leu 23
    Request for sequence
  • Molecular Characterization
    Online(Leu 23 - Asp 257) AAA36755

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 26.0 kDa. The protein migrates as 35-45 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human TNFR2, His Tag (Cat. No. TN2-H5227) SDS-PAGE gel

Human TNFR2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Human TNFR2, His TagHuman TNFR2, His Tag (Cat. No. TN2-H5227) ELISA bioactivity

Immobilized ActiveMax® Human TNF-alpha, Tag Free, low endotoxin (HPLC-verified) (Cat. No. TNA-H4211) at 5 μg/mL (100 μL/well) can bind Human TNFR2, His Tag (Cat. No. TN2-H5227) with a linear range of 5-39 ng/mL (QC tested).

Protocol
Human TNFR2, His TagHuman TNFR2, His Tag (Cat. No. TN2-H5227) ELISA bioactivity

Immobilized ActiveMax® Human TNF-beta, Tag Free (Cat. No. TNB-H5214) at 5 μg/mL (100 μL/well) can bind Human TNFR2, His Tag (Cat. No. TN2-H5227) with a linear range of 2-62 ng/mL (Routinely tested).

Protocol
  • Background
    TNF RI is also known as the p60 or p55 TNFR) and TNF RII (the p75 or p80 TNFR) are two distinct type I transmembrane glycoproteins that bind TNF with highaffinity.Both RI and RII are prototypic members of the TNF receptor superfamily and have been designated TNFRSF1A and TNFRSF1B, respectively. Human TNF RII cDNA encodes a 461 amino acid (aa) residue precursor protein with a 22 aa putative signal peptide, a 235 aa extracellular domain, a 20 aa transmembrane domain and a 174 aa cytoplasmic domain. TNFRII is expressed in fetal brain. The protein is produced naturally as a soluble form (sTNFRII). The soluble receptor inhibits TNFα action by competing with cell surface receptors in binding TNFα, thereby blocking its biologic effects. TNFRII is strongly expressed at the cartilage–pannus junction, and plays a major role in a subset of families with multiple cases ofrheumatoid arthritis (RA). Further, high plasma levels of sTNFRII were significantly associated with increased incidence of coronary heart disease, independent of established cardiovascular risk factors, and seems to be useful for monitoring the inflammatory activity of sarcoidosis.
  • References
    • (1)Alicia SC, et al., 2005, Molecular and Cellular Biology, 25, No. 11: 4716-26.
    • (2)Ziegenhagen, MW., et al., 2000, Journal of Internal Medicine, 248(1): 33-41.
    • (3)Iris Shai, et al., 2005, Diabetes Care, Vol. 28, No. 6: 1376-1382.
    • (4)MacEwan, D.J., 2002, Cell Signal. 14:477.
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

ACROBiosystems治疗性抗体的功效不仅取决于Fab片段及其与靶抗原的结合活性,还取决于Fc片段及其与关键Fc受体的相互作用。Fc片段对FcRn的结合亲和力(FCGRT&B2M)将预测抗体的半衰期,而Fc片段与FcγRIIIa(CD16a)之间的结合亲和力将影响抗体引发ADCC的能力(抗体依赖性细胞介导的细胞毒性)。因此,必须在抗体工程化过程中针对一组受体测试候选人。ACROBiosystems提供了完整的重组Fc受体蛋白集合,包括它们的常见变体,以帮助您加快mAb的开发。