- SynonymSECTM1,K12
- SourceHuman SECTM1, His Tag (SE1-H5227) is expressed from human 293 cells (HEK293). It contains AA Gln 29 - Gly 145 (Accession # AAH17716).Predicted N-terminus: Gln 29Request for sequence
- Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 13.5 kDa. The protein migrates as 18-22 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>90% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human SECTM1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
- BackgroundSecreted and transmembrane protein 1 (SECTM1) is also known as Protein K-12, which is belongs to the SECTM family, which is Inducted by IFNG/IFN-gamma (at protein level). SECTM1 is detected at the highest levels in peripheral blood leukocytes and breast cancer cell lines. SECTM1 may be involved in thymocyte signaling.
- References
- (1)Slentz-Kesler K.A., et al., 1998, Genomics 47:327-340.
- (2)Lyman S.D., et al., 2000, J. Biol. Chem. 275:3431-3437.
- (3)Lam G.K., et al., 2005, J. Clin. Immunol. 25:41-49.
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