- SynonymKITLG,FPH2,KL-1,Kitl,MGF,SCF,SF,SHEP7,KL
- SourceBiotinylated Human SCF, Avitag,His Tag (SCF-H82E1) is expressed from human 293 cells (HEK293). It contains AA Glu 26 - Ala 190 (Accession # AAH69797).Predicted N-terminus: Glu 26Request for sequence
- Molecular Characterization
This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag.
The protein has a calculated MW of 21.7 kDa. The protein migrates as 26-38 kDa under reducing (R) condition (SDS-PAGE) due to different glycosylation.
- BiotinylationBiotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
- Biotin:Protein RatioThe biotin to protein ratio is 0.5-1 as determined by the HABA assay.
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>95% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Biotinylated Human SCF, Avitag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human CD117, Fc Tag (Cat. No. CD7-H5255) at 2 μg/mL (100 μL/well) can bind Biotinylated Human SCF, Avitag,His Tag (Cat. No. SCF-H82E1) with a linear range of 0.1-3 ng/mL (QC tested).
- BackgroundKit ligand (KITLG) is also known as stem cell factor (SCF), mast cell growth factor (MGF), steel factor (SF), which belongs to the SCF family, and is a widely expressed 28 - 40 kDa type I transmembrane glycoprotein. KITLG is the ligand for the receptor-type protein-tyrosine kinase KIT. SCF / MGF plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG / SCF binding can activate several signaling pathways. KITLG / SF Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG / SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG / SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG / SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1, 4, 5 - trisphosphate. KITLG / SCF acts synergistically with other cytokines, probably interleukins.
- References
- (1)Anderson DM, et al., 1991, Cell Growth Differ. 2 (8): 373–8.
- (2)Broudy VC, 1997, Blood 90 (4): 1345–64.
- (3)Blouin R, Bernstein A, 1993, In Freedman MH, Feig SA.Boca Raton: CRC Press.
- (4)Kent D, et al., 2008, Clin. Cancer Res. 14 (7): 1926–30.
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