- SynonymPDGF-BB,PDGF-B,FLJ12858,PDGF2,SIS,SSV,c-sis
- SourceHuman PDGF-BB, Tag Free (PDB-H4112) is expressed from E.coli cells. It contains AA Ser 82 - Thr 190 (Accession # P01127-1).Predicted N-terminus: MetRequest for sequence
- Molecular Characterization
Human PDGF-BB, Tag Free is a disulfide-linked homodimeric polypeptide chain containing 2×109 amino acids, and has a calculated MW of 12.4 kDa. The reducing (R) protein migrates as a 14 kDa polypeptide and the non-reducing (NR) disulfide-linked homodimer migrates as a 27-32 kDa protein.
- EndotoxinLess than 1.0 EU per μg by the LAL method.
- Purity
>90% as determined byreducing (R) and non-reducing (NR) SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in 0.085% TFA in 30% ACN. Normally trehalose is added as protectant before lyophilization.
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- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Human PDGF-BB, Tag Free on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized Human PDGF-BB, Tag Free (Cat. No. PDB-H4112) at 5 μg/mL (100 μL/well) can bind Human PDGFRB, Fc Tag (Cat. No. PDB-H5252) with a linear range of 0.039-0.625 μg/mL (QC tested).
Immobilized Human PDGF-BB, Tag Free (Cat. No. PDB-H4112) at 2 μg/mL (100 μL/well) can bind Human PDGF R alpha, His Tag (Cat. No. PDA-H52H7) with a linear range of 0.02-0.625 μg/mL (Routinely tested).
The bio-activity was determined by dose-dependent stimulation of the proliferation of mouse 3T3 cells. The ED50 was 0.5-20 ng/mL (Routinely tested).
- Citations
Teniposide regulates the phenotype switching of vascular smooth muscle cells in a miR-21-dependent manner
Authors: Hao Han,et al.
Journal: Biochem Bioph Res Co 43409
Application: Cell Culture
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- BackgroundPDGFs are mitogenic during early developmental stages, driving the proliferation of undifferentiated mesenchyme and some progenitor populations. During later maturation stages, PDGF signalling has been implicated in tissue remodelling and cellular differentiation, and in inductive events involved in patterning and morphogenesis. In addition to driving mesenchymal proliferation, PDGFs have been shown to direct the migration, differentiation and function of a variety of specialised mesenchymal and migratory cell types, both during development and in the adult animal. Other growth factors in this family include vascular endothelial growth factors B and C (VEGF-B, VEGF-C)which are active in angiogenesis and endothelial cell growth, and placenta growth factor (PlGF) which is also active in angiogenesis. PDGF plays a role in embryonic development, cell proliferation, cell migration, and angiogenesis. PDGF is a required element in cellular division for fibroblast, a type of connective tissue cell. PDGF is also known to maintain proliferation of oligodendrocyte progenitor cells. Platelet-derived growth factor subunit B is also known as PDGFB, FLJ12858, PDGF2, SIS, SSV, c-sis, is a member of the platelet-derived growth factor family. PDGFB can exist either as a homodimer (PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide (PDGF-AB), where the dimers are connected by disulfide bonds. Mutations in this gene are associated with meningioma.
- References
- (1)Hoch RV, Soriano P. 2003, Development 130 (20): 4769–4784.
- (2)Olofsson B, et al. 1996, Proc Natl Acad Sci U S A.,93(6):2576-81.
- (3)Joukov V, et al. 1996, EMBO J. 15 (2): 290–298.
- (4)Lei KJ, et al. 1993, Oncogene 8 (4): 925–931.
- (5)Ratner L, et al. 1985, Nucleic Acids Res 13 (14): 5007–18.
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