- SynonymNECTIN4,LNIR, PRR4, PVRL4
- SourceMouse Nectin-4, His Tag (NE4-M52Ha) is expressed from human 293 cells (HEK293). It contains AA Gly 31 - Ser 347 (Accession # Q8R007-1).Predicted N-terminus: Gly 31Request for sequence
- Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 36.2 kDa. The protein migrates as 38-43 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
- EndotoxinLess than 0.1 EU per μg by the LAL method.
- Purity
>95% as determined by SDS-PAGE.
- Formulation
Lyophilized from 0.22 μm filtered solution in PBS, Arginine, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
- Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
- Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
Mouse Nectin-4, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Mouse Nectin-4, His Tag (Cat. No. NE4-M52Ha) at 5 μg/mL (100 μL/well) can bind Human PVRL1 Protein, Fc Tag with a linear range of 39-625 ng/mL (QC tested).
- BackgroundNectin-4 (gene name PVRL4, poliovirus receptor-like 4) is a 66 kDa type I transmembrane glycoprotein belonging to the Nectin family of Ig superfamily proteins. Nectins are cell adhesion molecules that play a key role in various biological processes such as polarity, proliferation, differentiation and migration, for epithelial, endothelial, immune and neuronal cells, during development and adult life. Nectin-4 is a tumor-associated antigen in 50%, 49% and 86%o of breast, ovarian and lung carcinomas, respectively, mostly on tumors of bad prognosis. Its expression is not detected in the corresponding normal tissues.
- References
Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.
